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M7027

Sigma-Aldrich

Methyl cellulose

viscosity: 15 cP, BioReagent, suitable for cell culture

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€128.00
250 G
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Estimated to ship on14 April 2025


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100 G
€128.00
250 G
€282.00

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.75

€128.00


Estimated to ship on14 April 2025


Request a Bulk Order

biological source

synthetic (organic)

Quality Level

product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

viscosity

12-18 cP, 2 % in H2O(20 °C)

solubility

water: 50 mg/mL, clear to hazy

SMILES string

[*]OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO[*])O[C@@H](O[*])[C@H](O[*])[C@H]2O[*])[C@H](O[*])[C@@H](O[*])[C@@H]1O[*]

InChI key

YLGXILFCIXHCMC-JHGZEJCSSA-N

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General description

Methyl cellulose is obtained by etherification of cellulose. This commercial cellulose ether exhibits amphiphilic properties and original physicochemical properties. Methyl cellulose is enzyme resistant, non-digestible, non-allergic, and non-toxic.

Application

Methyl cellulose has been used:
  • as a vehicle for metformin drug during oral gavage
  • as a component of mammary epithelial cell growth medium for mammosphere culture
  • as a supplement in spheroid 3D co-cultures for spheroid invasion assays using hanging drop protocol

Methyl cellulose is used to create semi-solid matrices,[1][2] as a cell culture gel, for culture of colony forming cells[3][4] such as CFU-G; CFU-GM; cells and other cells used in toxicity assays. Low viscosity, not for plaquing assays or cloning.

Biochem/physiol Actions

Methyl cellulose finds applications in various industries such as in the food industry it is used as an emulsifier, texturing agent, thickener, and gelling additive. Methyl cellulose is also used in various cosmetics and personal care products such as hair shampoos, hair styling products, liquid soaps, body washes, lotions and creams, and toothpaste to yield their characteristic thick consistency. In addition, it serves as an additive for pharmaceuticals and is used in the production of capsules for nutritional supplements. Methyl cellulose is used as an alternative to glycerol in a cryopreservation medium. It is used along with culture supplements to enhance proliferation and differentiation in multiple or single lineage-committed hematopoietic progenitor cells. This cellulose derivative also has the potential to prevent the loss of adhesive properties and cell functions of various cell types including human skin fibroblasts, human melanoma cells, and mouse lung fibroblasts cultured in suspension.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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High throughput and automatic colony formation assay based on impedance measurement technique
Lei KF, et al.
Analytical and Bioanalytical Chemistry, 409(12), 3271-3277 (2017)
Barley beta-glucan accelerates wound healing by favoring migration versus proliferation of human dermal fibroblasts
Fuste NP, et al.
Carbohydrate Polymers, 389-398 (2019)
Chandler Warr et al.
ACS applied bio materials, 3(4), 2239-2244 (2020-05-30)
We report a non-cytotoxic resin compatible with and designed for use in custom high-resolution 3D printers that follow the design approach described in Gong et al., Lab Chip 17, 2899 (2017). The non-cytotoxic resin is based on a poly(ethylene glycol)
Large variability in the motility of spiroplasmas in media of different viscosities
Boudet JF, et al.
Scientific Reports, 8(1), 17138-17138 (2018)
Dissociated effect and Chemosensitive enhancement of tumor spheroids influenced by an electric field in a microdevice
Huang Chun-Hao, et al.
Biomedical Microdevices, 20(3), 70-70 (2018)

Protocols

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Questions

1–10 of 13 Questions  
  1. How to practically reach a 1% solution of Methylcellulose in cell culture media that can't be heated excessively?

    1 answer
    1. This product is supplied in powder form. The solubility in water is 50 mg/mL, resulting in a hazy solution. To prepare a 1% Methylcellulose solution in cell culture media from this stock solution, follow these steps:
      A 1% solution requires 1 g of solute per 100 mL of solution, equivalent to 10 mg/mL. The stock solution is 50 mg/mL. To achieve this, take 20 mL of the 50 mg/mL stock solution and dilute it to a total volume of 100 mL.

      Helpful?

  2. I would like to use methylcellulose in my cell culture media to optimize organoids in suspension. What methylcellulose product would be the most appropriate? And can I dissolve it directly into the cold media?

    1 answer
    1. The methylcellulose products available are not currently tested for use in organoid suspension. However, a previously available product, S3812, was an IMDM-based stem cell culture medium that included a methylcellulose component with a 4000 cps viscosity, at a concentration of 1%. This would correspond to product M0512. The solubility in cold medium has not been determined. Please see the link below to review the general methylcellulose datasheet which includes a viscosity comparison table:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/183/010/m6385pis.pdf

      Helpful?

  3. Can the M7027 pass through a 0.2um filter?

    1 answer
    1. Methyl cellulose has a high molecular weight, and the viscosity of methyl cellulose solutions can also impede its ability to pass through such fine filters, as it can clog the filter pores. For sterilization, a 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of solution.

      Helpful?

  4. Does this product need to be sterilized after dissolving for use in cell culture?

    1 answer
    1. Solutions of this material require sterilization for cell culture applications. A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to a portion of the methylcellulose falling out of the solution.

      Helpful?

  5. Hello sir, I need to know molar mass or molecular weight of this code of Methyl cellulose or tell me how can i calculate can you help me?

    1 answer
    1. The molecular weight is not analyzed on a lot to lot basis. The approximate molecular weight is 88,000 Da with methoxy substitution between 27.5 - 31.5% (weight).

      Helpful?

  6. Can I use this to make 0.5% solution in water which will be used as the solvent to dissolve neratinib and administrate to mice through oral gavage?

    1 answer
    1. This product can be solubilized in water at 50mg/ml which is a 0.5% solution. This product is tested for use in cell cultue, however it has not been tested for in vivo applications. Suitability for in vivo use will need to be validated by the end-user. See the link below for a general product information sheet for Methyl Cellulose. Note product M7140, which is the general research grade of this product. All details for M7140 will apply to M7027.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/785/m0512pis.pdf

      Helpful?

  7. How can I make a solution of Methyl cellulose, Product M7027?

    1 answer
    1. There are two methods for solubilizing methylcellulose. Please see the product insert for Product No. M0387 for the protocols.

      Helpful?

  8. How do I store a stock solution of Methyl cellulose, Product M7027?

    1 answer
    1. A sterile solution of methylcellulose can be stored at room temperature for up to 1 year.

      Helpful?

  9. What concentration of Methyl cellulose, Product M7027, is used for cell culture?

    1 answer
    1. Product No. M7027 is used at approximately 50 g/L in media to enhance viscosity of culture media.

      Helpful?

  10. How do I sterilize a solution of Methyl cellulose, Product M7027?

    1 answer
    1. A 0.5-1% solution of methylcellulose can be autoclaved using standard conditions (121 °C, 15 psi, 15-20 minutes). Higher concentrations may lead to chunks of methylcellulose falling out of solution.

      Helpful?

1–10 of 13 Questions  

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