M5438
Mimetic Red 2 Ligand Affinity Adsorbent
suspension
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Application
Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Polymerase from E. coli lysates as well as for the purification of other DNA polymerases.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
95.0 °F - closed cup
Flash Point(C)
35 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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S L Meyer et al.
The Biochemical journal, 314 ( Pt 2), 511-519 (1996-03-01)
Calpain I is a heterodimeric protein that is part of a family of calcium-activated intracellular cysteine proteases presumed to play a role in mediating signals transduced by calcium. Expression of bioactive recombinant human calpain I has been achieved using the
N C Bruce et al.
The Biochemical journal, 274 ( Pt 3), 875-880 (1991-03-15)
The NADP(+)-dependent morphine dehydrogenase that catalyses the oxidation of morphine to morphinone was detected in glucose-grown cells of Pseudomonas putida M10. A rapid and reliable purification procedure involving two consecutive affinity chromatography steps on immobilized dyes was developed for purifying
Sotirios Melissis et al.
Biotechnology journal, 2(1), 121-132 (2006-12-22)
The thermostable Thermus aquaticus DNA polymerase (Taq Pol) has been the key factor in transforming the initial PCR method into one with huge impact in molecular biology and biotechnology. Therefore, the development of effective affinity adsorbents for the purification of
Jeannie Horak et al.
Analytical and bioanalytical chemistry, 400(8), 2349-2359 (2010-12-22)
The new affinity-type Mimetic Ligand™ B14 was coupled with a 1,2-diaminoethane spacer (2LP) and a [1,2,3]-triazole spacer (TRZ) to three different support media. In addition to the agarose-based PuraBead and the polymethacrylate-type Fractogel, three new polymeric support media were introduced
S Melissis et al.
Journal of chromatography. A, 1122(1-2), 63-75 (2006-05-23)
The commercial availability of DNA polymerases has revolutionized molecular biotechnology and certain sectors of the bio-industry. Therefore, the development of affinity adsorbents for purification of DNA polymerases is of academic interest and practical importance. In the present study we describe
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