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M0325

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts, non-essential amino acids, ʟ-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture

Synonym(s):

EMEM, MEM

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

components

glucose: 1.0 g/L (Dextro)
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
Earle’s salts (5% CO2): yes
NaHCO3: 2.2 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It is used for the cultivation of a wide variety of cells grown in monolayers. MEM includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. May be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

Application

Minimum Essential Medium Eagle has been used as a culture medium for:
  • thyroid cancer cell lines
  • macrophage-like DH82 cells
  • vero CCL-81 cells

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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