K4143
PKCη, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
MG C26269, MG C5363, PKCL, PRK CL, PRKCH, nPKC-eta
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
Quality Level
product line
PRECISIO® Kinase
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
73-99 nmol/min·mg
mol wt
~73 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PRKCH(5583)
General description
PKCη (protein kinase C η) belongs to the protein kiinase C family. It is mainly expressed in the squamous cell epithelia.
Biochem/physiol Actions
PKCη (protein kinase C η) mainly participates in terminal differentiation of keratinocytes. It is responsible for arresting the cell cycle at G1 phase. It is also involved in phosphorylation of p21 and suppression of cdk2 (cyclin-dependent kinase 2) kinase activity by associating with cyclin E/cdk2/p21 complex, thereby regulating cell cycle. PKCη is upregulated in breast cancer and is associated with cell growth.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Deepanwita Pal et al.
Biochimica et biophysica acta, 1830(8), 4040-4045 (2013-04-09)
Protein kinase C (PKC) serves as the receptor for tumor-promoting phorbol esters, which are potent activators of conventional (c) and novel (n) PKCs. We recently showed that these activators induced selective upregulation of PKCη in breast cancer cells. The objective
M Kashiwagi et al.
Oncogene, 19(54), 6334-6341 (2001-02-15)
PKC is activated on the cell membrane by phospholipids, thereby transducing signals to intracellular pathways. We provide here another function of PKC, namely, regulating cell cycle by interaction with the cyclin E/cdk2/p21 complex. Among the 10 isoforms of PKC, PKCeta
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