FG1215

RPMI-1640 Medium

With stable glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture

• Synonym(s): Roswell Park Memorial Institute 1640 medium
• UNSPSC Code: 12352207
• NACRES: NA.71

Properties

• product name: RPMI-1640 Medium, With L-alanyl-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Quality Level: 400
• sterility: sterile-filtered; sterile
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: sodium pyruvate: no; NaHCO₃: yes; HEPES: no; phenol red: yes; stable glutamine: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Application

RPMI-1640 Medium has been used to culture murine fibroblast cells. It has also been used to culture human ovarian and rat substantia nigra cells.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable