F8381

Fructose-6-phosphate Kinase, Pyrophosphate-dependent from Propionibacterium freudenreichii (shermanii)

lyophilized powder, ≥4.0 units/mg protein

• Synonym(s): 6-Phosphofructokinase(pyrophosphate), Pyrophosphate: D-fructose-6-phosphate 1-phosphotransferase
• CAS Number: 55326-40-4
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 2.7.1.90 (BRENDA, IUBMB)
• MDL number: MFCD00131108
• Specific activity: ≥4.0 units/mg protein

Properties

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥4.0 units/mg protein
• composition: Protein, 5.0-25.0% biuret
• storage temp.: −20°C

Description

Application

FBP was used to study the kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii.

Biochem/physiol Actions

Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.

Physical form

Contains imidazole salts and stabilizer

Other Notes

One unit will convert 1.0 μmole of pyrophosphate and fructose 6-phosphate to fructose 1,6-diphosphate and inorganic phosphate per min at pH 7.4 at 30 °C.

These two pyrophosphate-dependent fructose-6-phosphate kinases differ in their response to the physiological activator fructose 2,6-diphosphate [Fru (2,6)-P₂]. The enzyme from Propionibacterium freudenreichii (F8381) reportedly is not affected by Fru (2,6)-P₂. The enzyme from potato tubers (F2258) is stimulated by Fru (2,6)-P₂ (half-maximal activation at 5.5 nM Fru (2,6)-P₂ for potato tuber enzyme).

Safety Information

• Pictograms: GHS08,GHS05
• Signal Word: Danger
• Hazard Codes: H314,H334,H360D
• Precautionary Statements: P202 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Faceshields, Gloves