F5303
Monoclonal Anti-Falkor/PHD1 antibody produced in mouse
clone FLK-49, purified immunoglobulin, buffered aqueous solution
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FLK-49, monoclonal
form
buffered aqueous solution
species reactivity
mouse
technique(s)
microarray: suitable
western blot: 0.5 μg/mL using total cell extracts from 293T cells transfected with Falkor-myc tagged
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Egln2(112406)
General description
Falkor is expressed at different levels in various tissues. It is localized in the nuclei of cells and encoded by 2Kb mRNA. Falkor is ubiquitously expressed in various mouse tissue with high level expression in testis and the lowest expression in spleen and skeletal muscle.
Monoclonal Anti-Falkor/PHD1 (mouse IgG2a isotype) is derived from the FLK-49 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. The PHD1 gene is mapped to human chromosome 19q13.2and exists in three isoforms.
Specificity
Monoclonal Anti-Falkor/PHD1 recognizes mouse Falkor/PHD1 (approx. 47 kDa).
Immunogen
synthetic peptide corresponding to amino acids 2-20 of falkor/PHD1.
Application
Monoclonal Anti-Falkor/PHD1 antibody is suitable for microarray and western blot at a concentration of 0.5μg/mL using total cell extracts from 293T cells transfected with Falkor-myc tagged.
Monoclonal Anti-Falkor/PHD1 antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.
Biochem/physiol Actions
EGLN2 (Egl nine homolog 2), also known as prolyl hydroxylase domain-containing protein 1 (PHD1), functions as an oxygen sensor in cells and mediates oxygen homeostasis. It inhibits nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB) signaling. Polymorphisms in the EGLN2 is implicated in various colorectal, hepatocellular and non-small cell lung cancer.
Falkor, a novel cell growth regulator, is speculated that the falkor protein is associated with suppression of cell growth and comprises of several putative phosphorylation sites for proteins such as protein kinase C and casein kinase II. Falkor has a significant homology to the C.elegans EGL-9. The human homolog of Falkor and EGL-9 is PHD1 (prolyl hydroxylase 1) which is involved in the regulation of HIF-1α. PHD1 function as dioxygenases that regulate HIF-1α by prolyl hydroxylation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hamid Bur et al.
Anticancer research, 38(1), 329-336 (2017-12-27)
Hypoxia-inducible factors (HIFs) and prolyl hydroxylase domain (PHD) proteins control cellular oxygen homeostasis and a wide range of other processes. We immunohistochemically assessed the expression of HIF1α, HIF2α, PHD1, PHD2 and PHD3 in 115 cases of classical Hodgkin's lymphoma, all
Mohammad Hashemi et al.
Asian Pacific journal of cancer prevention : APJCP, 19(4), 923-926 (2018-04-26)
It has been shown that a 4-bp insertion/deletion (ins/del) polymorphism of EGLN2 influences the risk of several cancers. However, to date, no study has inspected the impact of the 4-bp ins/del polymorphism on breast cancer (BC) risk. A case-control study
Jianhe Huang et al.
The Journal of biological chemistry, 277(42), 39792-39800 (2002-08-16)
Hypoxia-inducible factor (HIF) is a heterodimeric transcription factor induced by hypoxia. Under normoxic conditions, site-specific proline hydroxylation of the alpha subunits of HIF allows recognition by the von Hippel-Lindau tumor suppressor protein (VHL), a component of an E3 ubiquitin ligase
Dorothy M Ryan et al.
PloS one, 9(2), e88051-e88051 (2014-02-06)
Genome-wide association studies (GWAS) and candidate gene studies have identified a number of risk loci associated with the smoking-related disease COPD, a disorder that originates in the airway epithelium. Since airway basal cell (BC) stem/progenitor cells exhibit the earliest abnormalities
Neta Erez et al.
Oncogene, 21(44), 6713-6721 (2002-10-03)
A novel cell growth regulator, named Falkor, was identified using a functional approach to mammalian gene cloning, the Genetic Supressor Elements (GSE) method. In this screen, expression of the C-terminal domain of Falkor conferred cells with resistance to cisplatin-induced growth
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