F4024

Anti-Biotin–FITC antibody, Mouse monoclonal

clone BN-34, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Biotin
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: BN-34, monoclonal
• Application: IHC (p)
• Citations: 4

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: BN-34, monoclonal
• form: buffered aqueous solution
• storage condition: protect from light
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Biotin is an essential vitamin required by cells in living organisms or in cultures. An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin.

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Immunogen

Biotin-KLH conjugate

Application

May be substituted for avidin to decrease non-specific binding.

Monoclonal Anti-Biotin-FITC antibody produced in mouse has been used in the localization of the antigen with a primary antibody, addition of a biotinylated antibody to bind to primary antibody and finally application avidin-FITC.

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Biochem/physiol Actions

An immunoglobulin mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

Solution in phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
• Pictograms: GHS05
• Signal Word: Danger
• Hazard Codes: H318
• Precautionary Statements: P280 - P305 + P351 + P338
• Hazard Classifications: Eye Dam. 1