F0552

Fas Ligand from mouse

>95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder

• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD00285792

Properties

• recombinant: expressed in mouse NSO cells
• Quality Segment: 100
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: monomer calculated mol wt ~18 kDa, 28-32 kDa by SDS-PAGE
• impurities: endotoxin, tested
• UniProt accession no.: P41047
• storage temp.: −20°C
• Gene Information: mouse ... Fasl(14103)

Description

General description

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.[1]

Application

Fas Ligand (FASLG) from mouse has been used for-

• the induction of apoptosis in PC12 cells and
• the induction of migration in BV-2 murine microglial cells.[2][3]

Biochem/physiol Actions

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E.[4] In CD4⁺ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.[5]

Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Protein belonging to the tumor necrosis factor (TNF) family of cytokines; induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

Measured by its ability to induce apoptosis in Jurkat cells.

Other Notes

Mouse Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acid residues 132-279.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable