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Merck

E4142

ExtrAvidin®−Cy3™

buffered aqueous solution

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00163600
Form:
buffered aqueous solution

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conjugate

CY3 conjugate

form

buffered aqueous solution

extent of labeling

3-9 mol Cy3 per mol protein

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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This Item
E2886E2636P1291
ExtrAvidin®−Cy3™ buffered aqueous solution

Sigma-Aldrich

E4142

ExtrAvidin®−Cy3™

ExtrAvidin®−Peroxidase buffered aqueous solution

Sigma-Aldrich

E2886

ExtrAvidin®−Peroxidase

ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution

Sigma-Aldrich

E2636

ExtrAvidin® –Alkaline Phosphatase

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

P1291

Peroxidase Anti-Peroxidase Soluble Complex antibody produced in rabbit

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

technique(s)

dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

dry ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

conjugate

CY3 conjugate

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

ExtrAvidin®-Cy3™ is suitable for the following applications:
  • Immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a dilution of 1:100
  • Immunocytochemistry.[1]
  • Used for labeling.[2]

Biochem/physiol Actions

Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.[3] It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin and binds biotin with high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii.[4] ExtrAvidin® is covalently conjugated to Cy3 dye and is used for various techniques.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Affinity purified protein

Legal Information

Cy3 is a trademark of Cytiva
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000381973
0000343868
0000319720
0000343868
0000381973

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