DNASE70
On-Column DNase I Digestion Set
sufficient for 70 preparations
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Synonym(s):
Deoxyribonuclease I Set
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| Size/SKU | Availability | Price |
|---|---|---|
1 set | Check Cart for Availability | € 240,00 |
About This Item
grade
Molecular Biology
Quality Segment
form
liquid
usage
sufficient for 70 preparations
technique(s)
RNA purification: suitable
storage temp.
−20°C
General description
The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.
The term ′On-Column′ indicates that digestion is accomplished in-process for true convenience. Each of Sigma′s total RNA purification kits utilize a binding column for purification. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow. To review the complete protocol, see the Technical Bulletin for any of the Kits named above.
Application
Biochem/physiol Actions
Features and Benefits
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description sufficient for 70 preparations | description sufficient for 10 preparations | description - | description Native DNase I from bovine pancreas. |
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) DNA extraction: suitable | technique(s) - |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade - |
| form liquid | form liquid | form buffered aqueous glycerol solution | form lyophilized |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage sufficient for 70 preparations | usage sufficient for 10 preparations | usage - | usage - |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
WGK
WGK 3
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