CRISPR20
Lenti CRISPR Universal Non-Target Control#3 Plasmid DNA (LV04 vector)
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About This Item
NACRES:
NA.51
UNSPSC Code:
41106610
Pricing and availability is not currently available.
form
liquid
Quality Segment
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA )
application(s)
CRISPR
CRISPR
shipped in
dry ice
storage temp.
−20°C
General description
The Lenti CRISPR Non-Target Control#3 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments). This vector is useful as a negative control in experiments using CRISPR lentiviral clones. The lenti CRISPR non-target control uses a dual-component system consisting of U6-driven guide RNA (non-targeting) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.
Application
Functional Genomics/Screening/Target Validation
Other Notes
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP002).
Legal Information
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This Item | |||
|---|---|---|---|
| description - | description - | description Targets no known genes from any species | description Targets no known genes from any species |
| form liquid | form liquid | form - | form - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA ) | concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| application(s) CRISPR | application(s) CRISPR | application(s) - | application(s) - |
Storage Class
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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