Drug metabolism assays, hepatocyte studies, toxicological studies.
Culture Medium
DM-160 AU + 2mM Glutamine + 2% Foetal Bovine Serum (FBS) (10% for initial cell attachment after thawing).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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