Ma-Mel-45a

13012486, human skin, Epithelial

• UNSPSC Code: 41106514

Properties

• product name: Ma-Mel-45a, from human skin, 13012486
• biological source: human skin
• description: Human melanoma, metastasis
• growth mode: Adherent
• morphology: Epithelial
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Description

Ma-Mel-45a (ESTDAB-173) was established from the brain metastasis in a 39 year-old male patient with metastatic melanoma (unknown primary stage IV). The Ma-Mel-45a cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 10,11
D13S317: 8
D16S539: 12
D5S818: 12
D7S820: 9,13
THO1: 7,8
TPOX: 11
vWA: 16,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 1-3 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 5 x10,000 cells/cm² .

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.