FM-95

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 13012425, human skin, Fibroblastic

• UNSPSC Code: 41106514
• Biological source: human skin
• Growth mode: Adherent
• Morphology: Fibroblastic
• Manufacturer/tradename: ECACC

Properties

• biological source: human skin
• description: Human melanoma, metastasis
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 13012425-DNA-5UG, pkg of vial of cells 13012425-1VL
• growth mode: Adherent
• morphology: Fibroblastic
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Biochem/physiol Actions

FM-95 (ESTDAB-034) was established from a metastatic cutaneous malignant melanoma of a male patient. The FM-95 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,10
D16S539: 9,13
D5S818: 12,13
D7S820: 8,11
THO1: 9.3,9.3
TPOX: 8
vWA: 15,18

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 8 x10,000 cells/cm²

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.