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C7688

Chaperonin 60 from Escherichia coli

>95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

Synonym(s):

Cpn60, GroEL

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Size/SKUAvailabilityPrice
1 mg
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€ 550,00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
Form:
lyophilized powder
Assay:
>95% (SDS-PAGE)
Biological source:
Escherichia coli
Recombinant:
expressed in E. coli overproducing strain

€ 550,00


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biological source

Escherichia coli

Quality Segment

recombinant

expressed in E. coli overproducing strain

assay

>95% (SDS-PAGE)

form

lyophilized powder

technique(s)

electron microscopy: suitable, mass spectrometry (MS): suitable

suitability

passes test (Refolding (w/GroES))

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... HSPD1(3329)

General description

Research area: Cell Signaling

Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles.

Application

Chaperonin 60 from Escherichia coli has been used:
  • in mass spectroscopy
  • in cryo-electron microscopy imaging as control for testing particle distribution
  • as standard in infrared spectrum measurements
  • as a protein sample for stability assessment and characterization studies using differential mobility analysis (DMA) and cryo-electron microscopy (cryo-EM).

Biochem/physiol Actions

Apart from its role in facilitating protein folding, chaperonin 60 (Cpn60) serves as an extracellular signaling protein, showing functional similarities to certain proinflammatory cytokines. Furthermore, Cpn60 contributes to the inhibition of lipid accumulation and adipogenesis during the initial stages of cellular differentiation, thereby exerting anti-obesity effects.

Packaging

Package size based on protein content.

Physical form

Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.

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SAB4501464C7438T0910
description

>95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

description

affinity isolated antibody

description

≥95.0% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder

description

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

biological source

Escherichia coli

biological source

rabbit

biological source

Escherichia coli

biological source

Escherichia coli

assay

>95% (SDS-PAGE)

assay

-

assay

≥95.0% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

technique(s)

mass spectrometry (MS): suitable, electron microscopy: suitable

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000, immunohistochemistry: 1:50-1:100, immunofluorescence: 1:100-1:500

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

recombinant

expressed in E. coli overproducing strain

recombinant

-

recombinant

expressed in E. coli overproducing strain

recombinant

expressed in E. coli

suitability

passes test (Refolding (w/GroES))

suitability

-

suitability

-

suitability

-

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

essentially salt-free, lyophilized powder


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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