The protein does not contain a His-tag.
C6624
Carbonic Anhydrase II human
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
CA-II, CA2, Carbonic Anhydrase 2
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| Size/SKU | Availability | Price |
|---|---|---|
500 μg | Check Cart for Availability | € 584,00 |
About This Item
Specific activity:
≥5,000 units/mg protein
Recombinant:
expressed in E. coli
Concentration:
800-1000 μg/mL
recombinant
expressed in E. coli
Quality Segment
form
buffered aqueous solution
specific activity
≥5,000 units/mg protein
mol wt
large subunit 17-22 kDa, small subunit 10-12 kDa
concentration
800-1000 μg/mL
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CA2(760)
Application
Human carbonic anhydrase II has been used in a study to assess quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion. Human carbonic anhydrase II has also been used in a study to investigate a new scrubber concept for catalytic CO2 hydration.
Biochem/physiol Actions
Carbonic anhydrase is a zinc metalloenzyme that catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. It is required for renal acidification. Its absence can lead to osteoporosis, renal tubular acidosis, and cerebral calcification. Therefore, this enzyme is an important target for inhibitors with clinical applications in glaucoma, epilepsy and Parkinson′s disease. In addition, it is being explored as a potential target for obesity and cancer. CAII has a molecular mass of approximately 30 kDa and is primarily present in type II pneumocytes. Due to this unique location it has been speculated that CAII is involved in pulmonary functions such as regulation of fluid secretion and facilitation of CO2 elimation. Sulfonamides, sulfamates and sulfamides are potent inhibitors of CA.
Physical form
Supplied as a solution in 20 mM Tris, pH 7.5, with 150 mM NaCl.
Other Notes
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, buffered aqueous solution | description ≥90% (SDS-PAGE), recombinant, expressed in E. coli, ≥5,000 units/mg protein, buffered aqueous solution | description lyophilized powder, ≥2,000 W-A units/mg protein | description lyophilized powder, ≥2,000 W-A units/mg protein |
| specific activity ≥5,000 units/mg protein | specific activity ≥5,000 units/mg protein | specific activity ≥2,000 W-A units/mg protein | specific activity ≥2,000 W-A units/mg protein |
| Gene Information human ... CA2(760) | Gene Information - | Gene Information - | Gene Information - |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| concentration 800-1000 μg/mL | concentration - | concentration - | concentration - |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
Storage Class
10 - Combustible liquids
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