C-24400
Melanocyte Growth Medium M2
Basal Medium, 500 ml
Synonym(s):
Recommended with C-39420
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About This Item
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form
aqueous solution
packaging
pkg of 500 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Melanocyte M2 Basal Medium (supplements sold separately)
To form a complete medium, it should be combined with the media supplements: C-39420
To form a complete medium, it should be combined with the media supplements: C-39420
Application
Melanocyte Growth Medium M2 is a serum-free and PMA (Phorbol Myristate Acetate)-free Medium that was developed for the optimized in vitro cultivation of human melanocytes. It is also suitable for human pigment epithelial cells, neuronal cells, and melanoma cells. The medium is optimized for primary human cells, but can also be used for equine, porcine, mouse, and rat melanocytes.Recommended for:Normal Human Epidermal Melanocytes (NHEM), juvenile foreskin, cultured in M2 MediumNormal Human Epidermal Melanocytes (NHEM), adult donor, cultured in M2 Medium
Components
Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium (Ready-to-use): Includes phenol red-free Basal Medium and SupplementMixSupplementMix: Contains all media supplements premixed in one vial
Quality
All lots of PromoCell Melanocyte Growth Medium M2 are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage and Stability
Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C
Subculture Routine
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recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Primary Human Epidermal Melanocytes and optimized growth media support in vitro cultivation.
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