Pericytes are positive for CD146, PDGFRβ, αSMA, CD13, NG2, Desmin, and negative for CD31, CD34, CD45, and CD56.
C-12980
Human Pericytes from Placenta (hPC-PL)
500,000 cryopreserved cells
Synonym(s):
Pericyte Culture
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1 EACH
€1,530.00
About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Recommended Products
biological source
human placenta
packaging
pkg of 500,000 cells
morphology
( pericytes)
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
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Cell Line Origin
Placenta
Application
Human Pericytes are isolated from human placenta tissue and provided in a cryopreserved format. Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors called hemangiopericytomas. To date, pericytes have demonstrated the ability to differentiate into adipocytes, osteoblasts, fibroblasts, smooth muscle cells, and phagocytes (macrophages).
Quality
Rigid quality control tests are performed for each lot of Pericytes. The cells are tested for morphology, adherence rate, and viability. Furthermore, they are characterized by flow cytometric analysis for a comprehensive panel ofmarkers, e.g. CD31, CD34, CD105, and CD146. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
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Other Notes
Recommended Plating Density: 4000 cells per cm2Tested markers: CD146+, CD105+, CD31-, CD34-
Recommended products
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
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Protocols
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What markers are used to characterize pericytes?
1 answer-
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How are pericytes isolated?
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Pericyte isolation generally starts with enzymatic digestion of tissue with Trypsin EDTA, usually followed by the isolation of microvessel fragments via successive filtration steps. Pure pericyte cultures can be obtained through either positive or negative immuno-selection via the use of magnetic beads or flow cytometry using the antibody markers above.
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How long do pericytes grow in culture?
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PromoCell’s human pericytes derived from microvessels of the human placenta are cryopreserved at the end of secondary culture (P1) and after thawing are considered P2. The number of populations doublings is not determined for each individual cell lot, however they are guarenteed to be grown for at least 15 population doublings.
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