A7437

Acetate Kinase from Escherichia coli

lyophilized powder, ≥150 units/mg protein (biuret)

• Synonym(s): ATP:Acetate phosphotransferase
• CAS Number: 9027-42-3
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54
• EC Number: 2.7.2.1 (BRENDA, IUBMB)
• MDL number: MFCD00130330
• Specific activity: ≥150 units/mg protein (biuret)

Properties

• form: lyophilized powder
• Quality Segment: 200
• specific activity: ≥150 units/mg protein (biuret)
• composition: Protein, 5.0-20.0%
• UniProt accession no.: P0A6A3
• foreign activity: adenosine triphosphatase and NADH oxidase ≤0.01%
• storage temp.: −20°C
• Gene Information: Escherichia coli K12 ... ackA(946775)

Description

General description

Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily. It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.

Application

Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .

Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.

Biochem/physiol Actions

Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.

Involved in the metabolism of propanoate, pyruvate and taurine

Physical form

Lyophilized powder containing trehalose with small amounts of potassium phosphate, magnesium chloride, and dithiothreitol

Other Notes

One unit will phosphorylate 1.0 μmole of acetate to acetyl phosphate per min at pH 7.6 at 25 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable