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Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast blue BB salt

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About This Item

UNSPSC Code:
12352106
eCl@ss:
42010105
NACRES:
NA.47

Quality Level

500

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast blue BB. The resulting insoluble diffuse, blue dye deposit indicates sites of alkaline phosphatase activity.

Kit Components Only

Product No.
Description
  • Citrate Solution (915) 50 mL
  • FBB-Alkaline Solution (863) 10 mL
  • Naphthol AS-BI Alkaline Solution (861) 10 mL
  • Neutral Red Solution (N6264) 50 mL
  • Sodium Nitrite Solution (914) 10 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Supplementary Hazards

EUH071

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Certificates of Analysis (COA)

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Gregory M Cooper et al.
Tissue engineering. Part A, 16(5), 1749-1759 (2009-12-24)
The purpose of this study was to demonstrate spatial control of osteoblast differentiation in vitro and bone formation in vivo using inkjet bioprinting technology and to create three-dimensional persistent bio-ink patterns of bone morphogenetic protein-2 (BMP-2) and its modifiers immobilized
Ming-Tzu Tsai et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 27(9), 1169-1174 (2009-03-11)
Human mesenchymal stem cells (hMSCs) are a promising candidate cell type for regenerative medicine and tissue engineering applications by virtue of their capacity for self-renewal and multipotent differentiation. Our intent was to characterize the effect of pulsed electromagnetic fields (PEMFs)
Ankur Sharma et al.
Cytotechnology, 72(3), 489-497 (2020-03-04)
In the present study, we used a serum-free culture media to propagate goat putative spermatogonial stem cells (SSCs) and evaluated the effect of crucial growth factors on relative expression of some SSC markers and self-renewal related genes. The enriched SSCs
Ariana Darcy et al.
Bone, 50(6), 1294-1303 (2012-03-17)
Bone homeostasis can be compromised by an increase in osteoclast-mediated resorption and/or a decrease in osteoblast-mediated bone deposition. While many efforts have focused on treating osteoclast resorption, there has been less emphasis on identifying strategies for promoting osteoblast function. Herein
S A Kuznetsov et al.
The Journal of cell biology, 153(5), 1133-1140 (2001-05-31)
We report the isolation of adherent, clonogenic, fibroblast-like cells with osteogenic and adipogenic potential from the blood of four mammalian species. These cells phenotypically resemble but are distinguishable from skeletal stem cells found in bone marrow (stromal stem cells, "mesenchymal
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