53366
Anti-Mouse IgG−Abberior® STAR 488 antibody produced in goat
for STED application
Synonym(s):
Abberior® STAR 488-Anti--Mouse−IgG antibody produced in goat
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
biological source
goat
Quality Segment
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse
availability
available only in USA and Canada
concentration
~1 mg/mL
fluorescence
λex 503 nm; λem 524 nm in PBS, pH 7.4
storage temp.
−20°C
General description
Abberior STAR 488 was developed for STED and confocal microscopy in the green spectral region. It is a bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line microscopes and Leica STED microscopes. It can be very effectively excited with the prominent 488 nm laser line. For STED microscopy, Abberior STAR 488 can be efficiently used with STED Laser wavelengths between 590 - 610 nm. Abberior STAR 488 can substitute dyes like Oregon Green 488, Atto™ 488 or Alexa Fluor™ 488. Abberior STAR 488 is the ideal partner for Abberior STAR 440SXP to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 503 (PBS pH 7.4), 504 (H2O), 507 (MeOH + 0.1% TFA)
Extinction Coefficient, εmax [M-1cm-1]: 65 000 (PBS pH 7.4), 80 000 (H2O), 85 000 (MeOH + 0.1% TFA)
Correction Factor, CF260 = ε260/εmax: 0,28 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,14 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 524 (PBS pH 7.4), 525 (H2O), 531 (MeOH + 0.1% TFA)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,89 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,9 (PBS pH 7.4)
Application
Designed and tested for fluorescent super-resolution microscopy
Analysis Note
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
Other Notes
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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53366, Anti-Mouse IgG-Abberior STAR 488
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Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We
S W Hell et al.
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Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported