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30693

Chromeo P503

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1 mg
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€ 536,00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352108

€ 536,00


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Quality Segment

form

lyophilized powder, solid

technique(s)

protein detection: suitable

color

blue to very dark blue

solubility

DMF: 1 mg/mL, clear, blue

fluorescence

λex 503 nm; λem 610 nm±20 nm in 0.1 M bicarbonate buffer pH 8.3

storage temp.

−20°C

General description

Chromeo P503 labels proteins and peptides by exhibiting a color change from blue to red upon binding to primary amines. Chromeo P503 displays a weak fluorescence with a quantum yield <1% in solution. After conjugation to a primary amine group, the label undergoes a shortwave spectral shift of >100 nm and the quantum yield rises to 50%. This property allows a distinct detection of primary amines, proteins, and other biomolecules.

Application

Chromeo P503 is used as a fluorogenic reagent to label primary amine groups within molecules such as proteins. Chromeo P503 labeled proteins may be studied in a variety of gel electrophoresis and chromatographic applications.

Legal Information

Chromeo is a trademark of Active Motif Chromeon GmbH

Disclaimer

To ensure stability, the lyophilized dye should be stored at 4°C in the dark. This product is guaranteed for 6 months from the date of arrival.

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This Item
114529P475821605
description

-

description

ACS reagent

description

pH indicator dye

description

suitable for microscopy

fluorescence

λex 503 nm; λem 610 nm±20 nm in 0.1 M bicarbonate buffer pH 8.3

fluorescence

-

fluorescence

-

fluorescence

-

color

blue to very dark blue

color

-

color

red to brown

color

blue to very dark blue

form

lyophilized powder, solid

form

powder

form

powder

form

powder

solubility

DMF: 1 mg/mL, clear, blue

solubility

water: 0.77 g/L at 100 °C

solubility

water: 1 mg/mL, opaque, dark red

solubility

methanol: water (1:1): 0.01 g/10 mL, blue to very deep blue

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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Questions

  1. What is the solubility of this product in Methanol?

    1 answer
    1. This compound is tested for solubility at 10 mg/mL in Dimethylformamide. Methanol and acetonitrile are also listed as solvents, but a rate has not been established. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/425/192/30693dat.pdf

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