16670

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

≥95.0% (HPLC), powder

• Synonym(s): BCIP^® p-toluidine salt, X-phosphate p-toluidine salt
• Empirical Formula (Hill Notation): C₈H₆BrClNO₄P · C₇H₉N
• CAS Number: 6578-06-9
• Molecular Weight: 433.62
• NACRES: NA.83
• PubChem Substance ID: 57647586
• UNSPSC Code: 12352204
• EC Number: 229-506-1
• MDL number: MFCD00040636

Properties

• Product Name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, ≥95.0% (HPLC)
• Quality Segment: 200
• assay: ≥95.0% (HPLC)
• form: powder
• storage temp.: 2-8°C
• SMILES string: Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23
• InChI: 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
• InChI key: QEIFSLUFHRCVQL-UHFFFAOYSA-N

Description

General description

Histochemical substrate for alkaline phosphatase.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

For the colorimetric detection of alkaline phosphatase-labeled molecules.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• ppe: dust mask type N95 (US), Eyeshields, Gloves
• pictograms: GHS06,GHS08,GHS09
• signalword: Danger
• hcodes: H301 + H311 + H331,H315,H317,H319,H351,H400
• pcodes: P273 - P280 - P301 + P310 + P330 - P302 + P352 + P312 - P304 + P340 + P311 - P308 + P313
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1A
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects