N6876

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

• Synonym(s): 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
• Linear Formula: C₄₀H₃₀N₁₀O₆ · 2Cl
• CAS Number: 298-83-9
• Molecular Weight: 817.64
• Beilstein: 4115923
• MDL number: MFCD00012159
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897795
• NACRES: NA.47

Properties

• Quality Level: 200
• Assay: ≥90.0% (HPLC)
• form: powder
• impurities: ≤10% solvent
• solubility: H₂O: 10 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• SMILES string: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChI key: FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

Application

• Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.[1]
• It has been used as a substrate for western blot detection.[2]
• It has been used for the staining of histological tissue sections.[3]

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.[4][5]

Substrates

Substrate for dehydrogenases[6] and other oxidases.[7]

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Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H302,H315,H319,H335,H371
• Precautionary Statements: P260 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P311
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2 - STOT SE 3
• Target Organs: Eyes,Central nervous system, Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable