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F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Synonym(s):

Ready-to-use density gradient media

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25 ML
€75.00
50 ML
€127.00

€75.00

Estimated to ship on13 April 2025

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25 ML
€75.00
50 ML
€127.00

About This Item

CAS Number:
26873-85-8
MDL number:
MFCD00081599
UNSPSC Code:
12352207
NACRES:
NA.75

€75.00

Estimated to ship on13 April 2025

Request a Bulk Order

sterility

0.2 μm filtered

Quality Level

200

type

Type 400

form

liquid

concentration

20% in H2O

technique(s)

titration: suitable

color

clear colorless

solubility

water: soluble

density

1.055-1.075 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

General description

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells[1]
  • Isolation of CD133 negative cells[2]
  • Isolation and expansion of circulating endothelial progenitor cells.[3]

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000347993
0000347992
0000347993
SLCQ8241
SLCM9158

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Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Junchao Xing et al.
Tissue engineering. Part C, Methods, 25(3), 137-147 (2019-02-09)
Patient-specific individual tissue-engineered bones (iTEBs) have been recognized as a promising strategy for treating large bone defects. However, current construction protocols of iTEBs vary between lots and lack standardization and quality control, hampering further research and application. This study was
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
Feizi F, et al.
Physical Chemistry, 1(1) (2014)
View All Related Papers

Protocols

Hematopoietic Stem Cell Culture Methods

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Questions

1–7 of 7 Questions  

  1. What are the differences between Ficoll, Ficoll Paque Plus, Ficoll Paque Premium, Histopaque and the different products with different densities?

    1 answer

    1. 1. Ficoll-Paque PREMIUM products - Sterile, ready-to-use density gradient media for the preparation of mononuclear cells - Based on Ficoll-Paque PLUS - Used for isolating high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood
      2. Regulatory Status - Neither Ficoll-Paque Plus nor Ficoll-Paque Premium products are approved for In Vitro Diagnostic Use - For clinical studies, a GMP grade product (Ficoll-Paque Premium) is preferred over a product produced under ISO 9001 - Both products are sterile and low in endotoxins
      3. Differences between Ficoll and Histopaque - Ficoll and Histopaque are essentially the same formulation - Ficoll is a trademark name for polysucrose 400, and Paque is a trademark name for sodium diatrizoate - Both can be used to separate various cell types based on different densities and species
      4.Cell Separation Using Ficoll and Histopaque - Different densities are used for different cell types and sometimes the same cell types, but from different species - Ficoll and Histopaque can be used to separate a variety of cell types based on different densities and species

      Helpful?

  2. Is Product F5415, Ficoll® solution 20%, sterile?

    1 answer

    1. No claims are made for sterility for F5415.  If a sterile product is required for cell culture use,  product F8636 has been gamma irradiated and is considered a sterile product.  Product F8636 is a powder and does not come as a sterile solution.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the difference between Product F5415, Ficoll® solution 20%, and the polysucrose type 400 used in the Histopaque 1077-1?

    1 answer

    1. Ficoll is a registered trademark. The trademark was formerly held by Pharmacia, but today Amersham Biosciences/GE Healthcare hold the trademark.  Since Sigma Aldrich uses polysucrose type 400 in the preparation of the various Histopaque products, the name polysucrose 400 is utilized.

      Helpful?

  5. Can more Ficoll be added to Product F5415, Ficoll® solution 20%, to increase the density to 1.077 g/mL?

    1 answer

    1. This is not recommended.  Ficoll has a molecular weight in the range of 400,000.  Even 20% solutions of such high molecular weight products have extremely low osmolality.  Without the addition of other lower molecular weight chemical, solutions of 20%+ of Ficoll are considered extremely hypotonic.  Cells exposed to hypotonic solutions would not maintain viability and are prone to bursting.

      Helpful?

  6. Is Product F5415, Ficoll® solution 20% suitable for the separation of mononuclear cells without further dilution?

    1 answer

    1. No.  The 20% solution of Ficoll has a density of approximately 1.066 to 1.068 g/mL.  Typically products such as Histopaque 1077-1 or Ficoll Paque have a density of approximately 1.076 to 1.078 g/mL.  Such ready to use solutions are also osmotically balanced by the addition of a lower molecular weight chemical.  Properly balanced solutions will typically have an osmolality in the range of approximately 280-300 mOsm/Kg. If a ready made product is required for the separation of mononuclear cells, products Histopaque 1077-1 or H8889 should be utilized.

      Helpful?

  7. Is there a Product Information Sheet for Product F5415, Ficoll® solution 20%?

    1 answer

    1. No.  However there is a Product Information Sheet for product F4375.  This product information sheet gives general information regarding Ficoll and possible uses.  If mononuclear cells are to be separated, a better choice of products is Histopaque 1077-1, H8889 or one of the prefilled Accuspin tubes.

      Helpful?

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