89397

Girard′s reagent T

derivatization grade (HPLC), LiChropur^™, 99.0-101.0% (AT)

• Synonym(s): (Carboxymethyl)trimethylammonium chloride hydrazide, (Hydrazinocarbonylmethyl)trimethylammonium chloride, Acethydrazide trimethylammonium chloride
• Linear Formula: [(CH₃)₃N⁺CH₂CONHNH₂]Cl^-
• CAS Number: 123-46-6
• Molecular Weight: 167.64
• UNSPSC Code: 12352200
• NACRES: NA.22
• PubChem Substance ID: 329769730
• EC Number: 204-629-3
• Beilstein/REAXYS Number: 3727163
• MDL number: MFCD00012009

Properties

• grade: derivatization grade (HPLC)
• Quality Segment: 100
• assay: 99.0-101.0% (AT)
• form: crystals
• quality: LiChropur^™
• technique(s): HPLC: suitable
• mp: 188-192 °C (dec.) (lit.)
• SMILES string: [Cl-].C[N+](C)(C)CC(=O)NN
• InChI: 1S/C5H13N3O.ClH/c1-8(2,3)4-5(9)7-6;/h4,6H2,1-3H3;1H
• InChI key: YSULOORXQBDPCU-UHFFFAOYSA-N

Description

Application

Girard′s reagent T may be used as a derivatizing reagent for the determination of 5-Formyl-2′-deoxyuridine in cellular DNA samples using liquid chromatography–tandem mass spectrometry (LC–MS/MS) technique. Reducing saccharides can be derivatized by Girard′s reagent T and this can be analyzed by matrix-assisted laser desorption/ionization coupled to time of flight mass spectrometry (MALDI-TOF-MS) technique.

Features and Benefits

• Girard′s reagent T is a chemical derivatization reagent for LC-MS.
• It has carbonyl functional group possessing high proton (cation) affinity that stabilizes a positive charge.       
• It is commonly used in the derivatization of nucleosides.        
• Derivatization reactions in mass spectrometry are used to improve ionization efficiency.
• Derivatization can enhance precise quantitative analysis for profiling of relatively small analyte molecules, particularly in metabolomics.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable