HybriScan™I kits are intended for the identification and result confirmation of microorganisms using rRNA as the detection target. Several customers also use it to detect microorganisms in food, beverage and water samples. The principle is based on in situ sandwich hybridization with two specific probes. As the rRNA of dead cells is degraded in the presence of RNase, only living cells are detected. Each cell has several thousand rRNA copies, so neither PCR nor a polymerase is needed for this technology, which means that this molecular biology test is not inhibited by the sample matrix. We recommend a 24-hour enrichment in Tomato Juice Broth (Cat. No. 17218) or Brettanomyces Selective Broth (Cat. No. MHA00BSM2)
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