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70191

Millipore

Mueller Hinton Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

MH agar; Antibiotic sensitivity test media; disc diffusion test media

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74

sterility

non-sterile

Quality Level

200

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 17.0 g/L
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

clinical testing
food and beverages
microbiology

storage temp.

10-30°C

suitability

nonselective for Neisseria spp.
nonselective for bacteria (General Media)

Related Categories

General description

Mueller Hinton Agar is specially designed for antibiotic susceptibility testing using the disc diffusion method (Kirby Bauer method). It has been recommended by the CLSI as the ideal medium for antibiotic susceptibility testing for the following reasons:

  • It shows good reproducibility from batch to batch
  • It is low in certain inhibitors like trimethoprim, sulfonamide and tetracycline
  • It supports the growth of both fastidious and non-fastidious bacterial pathogens
  • The starch in the media absorbs toxins thus preventing them from interfering with the antibiotics
  • It is a loose agar and therefore facilitates better diffusion of antibiotics
Mueller Hinton Agar has been used:
  • In culturing Escherichia coli ATCC™ 25922 strain for disc diffusion studies
  • In disk diffusion assay of fusidic acid against Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli
  • In agar diffusion assay of drug-loaded nano-hydroxyapatite formulations against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli

Application

Mueller Hinton Agar is a solid medium originally designed for the isolation of pathogenic Neisseria species, now widely used for antibiotic susceptibility testing (including sulfonamides) of aerobic and facultatively anaerobic bacteria isolated from clinical specimens.

Preparation Note

Suspend 38 g in 1 litre of distilled water, bring to the boil to dissolve the medium completely and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Enhancement of cefotoxamine and other cephalosporins against Enterococcus faecalis by blood supplemented Mueller-Hinton agar

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6979
BCCL0692
BCCK3298
BCCJ4445
BCCG9561

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Multi-drug delivery system based on lipid membrane mimetic coated nano-hydroxyapatite formulations
Placente D, et al.
International Journal of Pharmaceutics, 548(1), 559-570 (2018)
Preparation and evaluation of QbD based fusidic acid loaded in situ gel formulations for burn wound treatment
Aksu NB, et al.
Journal of drug delivery science and technology, 52, 110-121 (2019)
Solid-state structure and antimicrobial and cytotoxicity studies of a cucurbit [6] uril-like Cu6L4 constructed from 3, 5-bis [(1H-tetrazol-5-yl) methyl]-4H-1, 2, 4-triazol-4-amine
Vasylevskyi S, et al.
Acta Crystallographica Section C, Structural Chemistry, 74(11) (2018)
R D Jenkins et al.
Journal of clinical microbiology, 22(3), 369-374 (1985-09-01)
Disk diffusion susceptibility tests for enterococci are frequently modified by adding 5% sheep blood (SB) to Mueller-Hinton agar; the performance standards from the National Committee for Clinical Laboratory Standards sanction this addition. Susceptibility testing of aminoglycoside antibiotics is not recommended
G M Eliopoulos et al.
Diagnostic microbiology and infectious disease, 12(2), 149-156 (1989-03-01)
The activities of cefotaxime and other aminothiazoyl oxime cephalosporins against Enterococcus faecalis were enhanced by addition of 5% sheep blood to Mueller-Hinton agar. This effect was not seen with aztreonam (aminothiazoyl oxime monobactam), cefotiam (aminothiazoyl, nonoxime), or other cephalosporins, and
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