10602370001

Plasmin, bovine

from bovine plasma

• Synonym(s): Plasmin
• Enzyme Commission number: 3.4.21.7 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine plasma
• form: suspension
• specific activity: ≥2 units/mg protein (At 25 °C with Chromozym PL as the substrate.)
• mol wt: M_r ~85 kDa
• packaging: pkg of 1 mL (5 U)
• manufacturer/tradename: Roche
• optimum pH: 8.9
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Plasmin is derived from the proteolytic cleavage of its precursor plasminogen. Human Plasmin is made up of two polypeptide chains. The polypeptide chains are connected via disulfide bridges.

Specificity

Serine endopeptidase that hydrolyzes peptide and ester bonds at the carboxylic side of Lys or Arg; more selective than trypsin.

Application

Plasmin from bovine has been used as a standard for assessing plasmin biosensors and for proteolytic assays.

Biochem/physiol Actions

Plasmin plays an important role in proteolysis and fibrinolysis. It possesses inflammatory activity. Plasmin is also involved in the hydrolysis of peptide bonds following arginine or lysine. It also cleaves hormones like glucagon, thrombopoietin and growth hormones. Plasmin plays a role in cleavage of complement factors like C3 and C5, coagulation factors V and VIII, matrix proteins, growth factors, E-cadherins and metalloproteinase.

Physical form

Suspension in 3.2 M ammonium sulfate solution

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash