700 mm Hg (27.5” Hg, 0.9 bar) differential vacuum at 25 °C (77 °F)
About This Item
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material
polyethersulfone membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing
Quality Level
product line
Stericup®
feature
hydrophilic
packaging
pack of 12 ea
greener alternative product characteristics
Waste Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
parameter
max. temp. at 45 °C
description
2.4 mL (hold-up volume)
technique(s)
sterile filtration: suitable
H
188 mm
capacity
500 mL
diam.
9.3 cm
filter capacity
500 mL
filter diam.
73 mm
filtration area
40 cm2
matrix
Express PLUS™
pore size
0.22 μm pore size
fitting
inlet thread(s), 45 mm thread
greener alternative category
shipped in
ambient
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This Item | |||
|---|---|---|---|
| material polyethersulfone membrane, polystyrene funnel(s), polystyrene bottle, polystyrene housing | material polyethersulfone membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing | material polyethersulfone membrane, polystyrene bottle, polystyrene funnel(s), polystyrene housing |
| sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated | sterility sterile; irradiated |
| pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size | pore size 0.22 μm pore size |
| packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea | packaging pack of 12 ea |
| fitting inlet thread(s), 45 mm thread | fitting inlet thread(s), 38 mm (1 1/2 in.) thread | fitting inlet thread(s), 38 mm (1 1/2 in.) thread | fitting inlet thread(s), 38 mm (1 1/2 in.) thread |
| feature hydrophilic | feature hydrophilic | feature hydrophilic | feature hydrophilic |
General description
The Stericup® E and Steritop® E sterile filters are designed as a Greener Alternative for vacuum sterile filtration that significantly reduces plastic and packaging.
This Stericup® E is a 45 mm thread collar which uses a 0.22 μm pore size polyethersulfone (PES) membrane filter unit combined with a 500 mL receiver flask and cap for processing and storage. The 45 mm thread directly connects to wider neck media bottles (such as Gibco® or glass bottles).
Use only glass or plastic bottles designed for vacuum applications.
Application
- Tissue culture media +/- additives
- Buffers
- Biological solutions
Features and Benefits
- The filter collar disconnects from the receiver bottle with just a quarter turn—reducing the likelihood of spillage and making it easier to manipulate on the bench or under the hood.
- Bold product identification on the filter collar plainly displays membrane characteristics, including composition and pore size, for rapid, application-appropriate product selection.
- Click seal cap is flanged and modified to enable a firm grip in wet or dry conditions—and a tactile stop confirms secure closure. Worry less about the possibility of spilling or contamination of sterilized contents.
- Frosted, ample writing surface on the receiver bottle and lighter cap color facilitate clear labelling to improve legibility and reduce unreadable smears.
- Click seal cap rests on the side to avoid risk of contamination.
- Receiver bottles are stackable to save storage space.
Legal Information
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Certificates of Analysis (COA)
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Articles
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Related Content
Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.
Stericup® E and Steritop® E sterile filters help reduce laboratory environmental impact by significantly reducing plastic and packaging waste. Both attach directly to commercial media bottles, eliminating the plastic funnel while they eliminate contaminants from media and buffers.
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What is the maximum vacuum pressure for filtration?
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Are Stericup® and Steritop® filters GMP-certified?
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No, Stericup® and Steritop® devices are not GMP-approved.
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What leachables are detected during filtration processes using Stericup® filters?
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We have not performed extractables/leachables studies for our Stericup® filters.
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Can Stericup® and Steritop® filters be used to prepare solutions that will be administered to patients?
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No, all Stericup® and Steritop® devices are Research Use Only.
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Which type of pump should be used with Stericup® and Steritop® vacuum filters?
Which type of pump should be used with Stericup® and Steritop® vacuum filters?
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We recommend our Chemical Duty Pump: https://www.sigmaaldrich.com/product/mm/wp6111560
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What tubing can be used with Stericup® and Steritop® vacuum filters?
What tubing can be used with Stericup® and Steritop® vacuum filters?
1 answer-
Stericup® and Steritop® filters are fitted with a 1/4” tube adapter. We recommend 3/16 in. (4.8 mm) ID silicone tubing to fit this tube adapter: https://www.sigmaaldrich.com/product/mm/xx7100004
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Can we filter oil-type liquids with Stericup® filters?
Can we filter oil-type liquids with Stericup® filters?
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Likely not, due to pore size/possible fouling/chemical compatibility. Refer to our guide, "Chemical compatibility of filter components": https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/302/855/membrane-chemical-compatibility-ds2532en-ms.pdf
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What is the maximum processing volume for Stericup® and Steritop® filters? Can I add more volume after initial filtration?
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All Stericup® and Steritop® filters were developed for single-use application. The maximum volume is dependent on the receiver bottle capacity. Stericup® filters come with a receiver bottle with a specified maximum volume. For Steritop® filters, the maximum recommended volume is 2 L with an appropriate-sized receiver bottle. Larger processing volumes can result in membrane fouling, increase chance of spillage and contamination, and lead to possible airlock*.
*Note that devices with PES membranes can airlock if filtered to dryness. Some liquid must be left on top of the filter, then the system stopped, receiver changed or emptied, and more sample volume added before restart. This increases the chance of spillage.Helpful?
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What is the difference between classic Stericup® filters and Stericup® “Quick Release” filters?
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Stericup® Quick Release, or “QR”, filters feature a connection between funnel filter and receiver bottle that requires only a quarter-turn release to facilitate funnel removal. These filters come fully engaged – there is no need to tighten before use. The receiver bottle cap has flanges on the top surface to improve grip, as well as a positive lock stop to ensure a leak-proof seal with the receiver bottle. Other improvements include a large, frosted writing surface on the receiver bottle and a lighter cap color to facilitate labelling, more prominent volume markings on the filtration funnel and receiver bottle, and a product label with a peel-off lot record strip on the bag for easy viewing in storage.
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What Bacterial Endotoxins Test is used?
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The test method used complies with USP 85 and EP 2.6.14 to ensure the absence of endotoxin in our products.
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