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SCM306

Sigma-Aldrich

Anterior Foregut Endoderm Induction Medium II

Serum-free media used to differentiate definitive endoderm (DE) cells into anterior foregut endoderm (AFE) cells.

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

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General description

The Anterior Foregut Endoderm Induction Medium II is a chemically defined/serum-free ready-to-use media used to differentiate CXCR4+/c-kit+ definitive endoderm (DE) cells into Sox2+/Pax6+ anterior foregut endoderm (AFE) cells. The Anterior Foregut Endoderm Induction Medium II is a component of the 3dGRO® Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. The 3dGRO® Human Lung Organoid Culture System includes (all sold separately):

  • Definitive Endoderm Induction Medium (SCM302)
  • Anterior Foregut Endoderm Induction Medium I (SCM305)
  • Anterior Foregut Endoderm Induction Medium II (SCM306)
  • 3dGRO® Lung Organoid Branching Medium (SCM307)
  • 3dGRO® Lung Organoid Maturation Medium (SCM308)

Human iPSC Derived AFE Progenitors (SCC301)

Storage and Stability

Upon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.

Legal Information

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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