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description
Neural Stem Cells
Quality Level
type
for cell culture
form
liquid
technique(s)
cell culture | stem cell: suitable
General description
The Mouse Neural Stem Cell Expansion Medium allows for the growth and proliferation of mouse neural stem cells. This product includes the Neural Stem Cell Basal Medium and the supplements bFGF, EGF, and Heparin. Withdrawal of the growth factors and heparin from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of mouse neural stem cells.
Components
500 mL of Neural Stem Cell Basal Medium (Cat.# SCM003) bFGF, 10 ug EGF, 10 ug Heparin, 1.5 mg
Quality
Each lot of medium is tested for the optimal cell growth, proliferation and differentiation of mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4
Storage and Stability
Part number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. bFGF, EGF and heparin solutions should be stored in working aliquots at -20°C for up to six months. The solutions can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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