SCC208
INS-1 832/3 Rat Insulinoma Cell Line
Rat
Synonym(s):
INS1 832/3, INS-1 (832/3), 832/3
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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product name
INS-1 832/3 Rat Insulinoma Cell Line, INS-1 832/3 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.
biological source
rat
technique(s)
cell culture | mammalian: suitable
shipped in
ambient
General description
Glucose-stimulated insulin secretion (GSIS) is potentiated by pancreatic beta cells and is critical to the physiological control of blood glucose levels. Insulin secretion is impaired in type 2 diabetes, and insight into the mechanisms and regulation of insulin secretion is fundamental to understanding the roles of beta cells in metabolic disease.
The rat insulinoma cell line INS-1 is a well-established model for studies of pancreatic islet beta-cell function ; however, the GSIS response of INS-1 cells may decrease over time. The INS-1 832/3 cell line is a subclone of INS-1 that was selected for robust GSIS, producing and secreting both rat and human insulin. INS-1 832/3 harbors a human insulin expression cassette allowing for human insulin secretion to be maintained over extended passages with selection . INS-1 832/3 cells are widely used to study the mechanisms of cellular insulin secretion, storage and synthesis. INS-1 832/3 cells may be characterized by granular staining for synaptotagmin, as described for the parental cell line . The INS-1 832/3 cell line exhibits the unique feature of additional insulin secretion in response to natural incretin hormones such as glucagon-like peptide 1 (GLP1), pituitary adenylate cyclase-activating peptide (PACAP), and gastric inhibitory peptide (PIP) , making INS-1 832/3 a valuable tool for physiologically relevant investigations of insulin regulation.
Source:
INS-1 832/3 is a derivative of INS-1 cells originally established from an x-ray induced insulinoma in rat1. The INS-1 832/3 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human insulin expression plasmid carrying a geneticin (G418)-resistance marker for selection .
Source:
INS-1 832/3 is a derivative of INS-1 cells originally established from an x-ray induced insulinoma in rat1. The INS-1 832/3 cell line is a subclone of INS-1 that was stably transfected with a CMV promoter-human insulin expression plasmid carrying a geneticin (G418)-resistance marker for selection .
Cell Line Description
Cancer Cells
Application
INS-1 832/3 rat insulinoma cell line is a useful model for insulin secretion regulation and pancreatic islet beta-cell function studies.
Research Category
Metabolism
Metabolism
Research Sub Category
Diabetes
Diabetes
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Quality
• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Disclaimer
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Sarah M Ronnebaum et al.
The Journal of biological chemistry, 283(43), 28909-28917 (2008-08-30)
We have previously demonstrated a role for pyruvate cycling in glucose-stimulated insulin secretion (GSIS). Some of the possible pyruvate cycling pathways are completed by conversion of malate to pyruvate by malic enzyme. Using INS-1-derived 832/13 cells, it has recently been
Tetraspanin-8 sequesters syntaxin-2 to control biphasic release propensity of mucin granules.
Wojnacki, et al.
Nature Communications, 14, 3710-3710 (2023)
Shu-Chen Lu et al.
Cell reports. Medicine, 2(5), 100263-100263 (2021-06-08)
Glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) regulate glucose and energy homeostasis. Targeting both pathways with GIP receptor (GIPR) antagonist antibody (GIPR-Ab) and GLP-1 receptor (GLP-1R) agonist, by generating GIPR-Ab/GLP-1 bispecific molecules, is an approach for treating obesity and
J Lang et al.
The EMBO journal, 16(19), 5837-5846 (1997-10-06)
The Ca2+- and phospholipid-binding protein synaptotagmin is involved in neuroexocytosis. Its precise role and Ca2+-affinity in vivo are unclear. We investigated its putative function in insulin secretion which is maximally stimulated by 10 microM cytosolic free Ca2+. The well-characterized synaptotagmin
H E Hohmeier et al.
Diabetes, 49(3), 424-430 (2000-06-27)
The biochemical mechanisms involved in regulation of insulin secretion are not completely understood. The rat INS-1 cell line has been used to gain insight in this area because it secretes insulin in response to glucose concentrations in the physiological range.
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