TGF-beta-activated kinase 1 and MAP3K7-binding protein 2, Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, TAB-2, TGF-beta-activated kinase 1-binding protein 2
TGF-beta-activated kinase 1 and MAP3K7-binding protein 2 (UniProt: Q9NYJ8; also known as Mitogen-activated protein kinase kinase kinase 7-interacting protein 2, TAK1-binding protein 2, TAB-2, TGF-beta-activated kinase 1-binding protein 2) is encoded by the TAB2 (also known as KIAA0733, MAP3K7IP2) gene (Gene ID: 23118) in human. TAB-2 is a peripheral membrane protein that serves as an adapter protein that links MAP3K7/TAK1 and TRAF6. Two isoforms of TAB-2 have been reported that are produced by alternative splicing. TAB-2 promotes MAP3K7 activation in the IL-1 signaling pathway. Following IL-1 stimulation it translocates from the membrane to cytosol. TAB-2 is widely expressed. In the embryo, it is expressed in the ventricular trabeculae, endothelial cells of the conotruncal cushions of the outflow tract and in the endothelial cells lining the developing aortic valves. TAB-2 contains a RanBP2-type zinc finger domain (aa 663-693) that mediates binding to two consecutive Lysine 63-linked ubituitins. The binding of Lysine 63-linked polyubiquitin chains to TAB-2 promotes autophosphorylation of MAP3K7 at Threonine 187. Mutations in TAB2 gene are reported to cause multiple congenital heart defects characterized by congenital developmental abnormalities involving structures of the heart, including left ventricular outflow tract obstruction, sub-aortic stenosis, atrial fibrillation, bicuspid aortic valve and aortic dilation.
Specificity
Clone 1D5.2 specifically detects human TGF-beta-activated kinase 1 and MAP3K7-binding protein 2. It targets an epitope within 133 amino acids from the C-terminal region.
Immunogen
GST/His-tagged recombinant fragment corresponding to 133 amino acids from the C-terminal region of human TAK1-binding protein 2 (TAB-2)
Application
Anti-TAB-2, clone 1D5.2, Cat. No. MABS1894, is a mouse monoclonal antibody that detects TAK1-binding protein 2 and has been tested for use in Western Blotting.
Western Blotting Analysis: 1 µg/mL from a representative lot detected TAB-2 in 10 µg of Raji cell lysate.
Quality
Evaluated by Western Blotting in TF-1 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected TAB-2 in 10 µg of TF-1 cell lysate.
Target description
~77 kDa observed; 76.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Other Notes
Concentration: Please refer to lot specific datasheet.
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