NOD1/CARD4, also known as Nucleotide-binding oligomerization domain-containing protein 1, or Caspase recruitment domain-containing protein 4, and encoded by the gene NOD1/CARD4, is an important apoptosis mediating protein that enhances caspase-9-mediated apoptosis. NOD1/CARD4 also mediates cellular responsiveness to intracellular bacterial LPS and forms an intracellular sensing system along with another protein called ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. NOD1/CARD4 is a homodimer that is found in the cytoplasm but also associated with the cell membrane in particular cells such as polarized epithelial cells. NOD1/CARD4 is expressed in many tissues including heart, skeletal muscle, and pancreas. As part of the innate immune system NOD1/CARD4 is part of a family of proteins that are responsible for the initial response of an organism to potentially harmful stressors, pathogens or tissue injury, and accordingly plays an essential role in the pathogenesis of many inflammatory processes, including some cardiovascular diseases.
Immunogen
Epitope: CARD domain
Recombinant protein corresponding to the CARD domain of human NOD1/CARD4.
Application
Anti-NOD1/CARD4 Antibody, clone 021 is an antibody against NOD1/CARD4 for use in western blotting & flow cytometry.
Research Category Inflammation & Immunology
Research Sub Category Immunoglobulins & Immunology
Target description
~125 kDa observed. The calculated molecular weight of this protein is 108 kDa, but has been observed at ~125 kDa in some cell lysates. An IgG heavy chain may be observed at 55 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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