MABE666

Anti-Heterochromatin Protein-1 Antibody, clone 4C12.1

clone 4C12.1, from mouse

• Synonym(s): Chromobox protein homolog 5, Antigen p25, HP1 alpha
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 4C12.1, monoclonal
• Application: IHC, WB
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4C12.1, monoclonal
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_001120793
• UniProt accession no.: P45973
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... CBX5(23468)

Description

General description

Chromobox protein homolog 5 (UniProt: P45973; also known as Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha) is encoded by the CBX5 (also known as HP1A) gene (Gene ID: 23468) in human. Chromobox protein homolog 5 is a component of centromeric and pericentromeric heterochromatin that associates with chromosomes during mitosis. Chromobox protein homolog 5 associates specifically with chromatin during metaphase and anaphase. It recognizes and binds histone H3 tails methylated at Lys9 (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when Tyr41 of histone H3 is phosphorylated. It can interact with lamin-B receptor (LBR) and this interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. It is also involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

~23 kDa observed; 22.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

GST/His-tagged recombinant fraction corresponding to 68 amino acids from the internal region of human Heterochromatin protein 1 homolog alpha.

Application

Anti-Heterochromatin Protein-1 , clone 4C12.1, Cat. No. MABE666, is a highly specific mouse monoclonal antibody that targets Heterochromatin Protein-1 alpha and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Heterochromatin Protein-1 in human tonsil, human pancreas, and human uterus tissues.

Research Category
Epigenetics & Nuclear Function

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Heterochromatin Protein-1 in 10 µg of NIH/3T3 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Replaces: MAB3584

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• wgk: WGK 1