MABE462

Anti-TET2 Antibody, clone hT2H 21F11

clone hT2H21F11, from mouse

• Synonym(s): Methylcytosine dioxygenase TET2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: hT2H21F11, monoclonal
• Application: ChIP, IP, WB
• Citations: 20

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: hT2H21F11, monoclonal
• species reactivity: human, mouse
• technique(s): ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001120680
• UniProt accession no.: Q6N021
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TET2(54790)

Description

General description

Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).

~ 260 kDa observed. The calculated molecular weight of this protein is 224 kDa, but can be observed at ~260 kDa

Immunogen

Recombinant protein corresponding to the N-terminus of human TET2.

Application

Detect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected TET2 in 10 µg of TF-1 cell lysate.
Immunoprecipitation Analysis: 5 µg of a representative lot immunoprecipitated TET2 in 250 µg of HL60 RIPA cell lysate.

Biochem/physiol Actions

This antibody recognizes the N-terminus of TET2

Physical form

Format: Purified

Analysis Note

Control
MOLT-4 cell lysate

Evaluated by Western Blotting in MOLT-4 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TET2 in 10 µg of MOLT-4 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable