ABN45
Anti-Myelin Regulatory Factor (MRF) Antibody
1.0 mg/mL, from rabbit
Synonym(s):
Myelin regulatory factor, Myelin gene regulatory factor, Myelin regulatory factor, N-terminal, Myelin regulatory factor, C-terminal
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
species reactivity (predicted by homology)
hamster (based on 100% sequence homology), rat (based on 100% sequence homology), monkey (based on 100% sequence homology), horse (based on 100% sequence homology)
concentration
1.0 mg/mL
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MYRF(745)
General description
Myelin Regulatory Factor (MRF), also known as Myelin regulatory factor, MYRF, and encoded by the gene MYRF/C11orf9/KIAA0954/MRF, is an interesting protein that gets cleaved into two chains. The N terminal component specifically activates transcription of myelin gene in the CNS oligodendrocytes and pre-oligodendrocytes. The C-terminal portion, which is remains membrane bound, appears to play no role in gene induction but its complete role is still being discovered. The N-terminal portion of MRF activates MBP, MOG, MAG, and PLP among other genes, but it is not required for oligodendrocyte differentiation but is required for the maintenance of myelin in the adult. MRF whole factor is localized to the endoplasmic reticulum. The cleaved N terminal portion translocates to the nucleus and the C-terminal portion remains in the endoplasmic reticulum. Interestingly MRF can be found expressed outside of oligodendrocytes and oligo precursors in the brain including regions such as the lung, basal ganglion and liver.
Immunogen
Epitope: cytoplasmic domain, N-terminal chain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Myelin Regulatory Factor (MRF).
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Myelin Regulatory Factor (MRF) Antibody is validated for use in western blotting & IHC for the detection of Myelin Regulatory Factor (MRF).
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Myelin Regulatory Factor (MRF) in 10 µg of ARPE19 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Myelin Regulatory Factor (MRF) in human cerebral cortex, human retina, and human cerebellum tissue.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Myelin Regulatory Factor (MRF) in human cerebral cortex, human retina, and human cerebellum tissue.
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myelin Regulatory Factor (MRF) in 10 µg of mouse brain tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Myelin Regulatory Factor (MRF) in 10 µg of mouse brain tissue lysate.
Target description
~160 kDa observed. The expected molecular weight is 125 kDa and ~160kDa when glycosylated. The protein was cleaved into a ~90 kDa N-terminal fragment from the full length of ~160 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Sanaz Gabery et al.
Acta neuropathologica, 142(5), 791-806 (2021-08-28)
Huntington disease (HD) is a fatal neurodegenerative disorder caused by an expanded CAG repeat in the huntingtin (HTT) gene. The typical motor symptoms have been associated with basal ganglia pathology. However, psychiatric and cognitive symptoms often precede the motor component
Ciara E Keogh et al.
Brain, behavior, and immunity, 91, 437-450 (2020-11-07)
Myelination in the peripheral and central nervous systems is critical in regulating motor, sensory, and cognitive functions. As myelination occurs rapidly during early life, neonatal gut dysbiosis during early colonization can potentially alter proper myelination by dysregulating immune responses and
Naomi Tanga et al.
Glia, 67(5), 967-984 (2019-01-23)
Protein tyrosine phosphatase receptor type Z (PTPRZ) maintains oligodendrocyte precursor cells (OPCs) in an undifferentiated state. The inhibition of PTPase by its ligand pleiotrophin (PTN) promotes OPC differentiation; however, the substrate molecules of PTPRZ involved in the differentiation have not
Liang Zhou et al.
eLife, 9 (2020-01-17)
Oligodendrocytes (OLs) myelinate axons and provide electrical insulation and trophic support for neurons in the central nervous system (CNS). Platelet-derived growth factor (PDGF) is critical for steady-state number and differentiation of oligodendrocyte precursor cells (OPCs), but its downstream targets are
Bang Li et al.
Cell discovery, 9(1), 27-27 (2023-03-07)
Autism spectrum disorder (ASD) is a complex neurodevelopmental condition that affects social interaction and behavior. Mutations in the gene encoding chromodomain helicase DNA-binding protein 8 (CHD8) lead to autism symptoms and macrocephaly by a haploinsufficiency mechanism. However, studies of small
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