ABF191

Anti-FcRn (FCGRT)

from rabbit

• Synonym(s): IgG receptor FcRn large subunit p51, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μL
• technique(s): immunofluorescence: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_034319
• UniProt accession no.: Q61559
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: mouse ... Fcgrt(14132)

Description

General description

IgG receptor FcRn large subunit p51 (UniProt: Q61559; also known as FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor) is encoded by the Fcgrt (also known as Fcrn) gene (Gene ID: 14132) in murine species. FcRn is a single-pass type I membrane protein that binds to the Fc region of monomeric immunoglobulins gamma. FcRn complex consist of two subunits: p51 and p14 and contains one Ig-like C1-type (immunoglobulin-like) domain of 88 amino acids. FcRn mediates the selective uptake of IgG from milk and helps newborn animals to acquire passive immunity. In rodents, FcRn functions effectively in the neonatal period when it transports maternally derived IgG in ingested milk across the epithelial-cell layer of the proximal small intestine. However, in humans it transports maternal antibody to the fetus across the placenta. In rodent FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. FcRn also protects IgG from degradation providing long half-life of this class of antibody in the serum. (Ref.: Roopenian,, DC and Shreeram,, A (2007). Nat. Rev. Immunol. 7(9); 715-725).

Specificity

This polyclonal antibody detects murine IgG receptor FcRn. It targets an epitope within 44 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus

GST-tagged recombinant fragment corresponding to 44 amino acids from the C-terminal region of murine IgG receptor FcRn large subunit p51.

Application

Immunofluorescence Analysis: A representative lot detected FcRn (FCGRT) in mouse uterus and vagina (Li, Z., et. al. (2011). Proc Natl Acad Sci USA. 108(11):4388-93).

Western Blotting Analysis: A representative lot detected FcRn (FCGRT) in mouse liver, mouse uterus, and vagina tissue lysate (Li, Z., et. al. (2011). Proc Natl Acad Sci USA. 108(11):4388-93).

Research Category
Inflammation & Immunology

This rabbit polyclonal Anti-FcRn (FCGRT) antibody, Cat. No. ABF191, detects IgG receptor FcRn large subunit p51. It has been tested for use in Immunofluorescence and Western Blotting.

Quality

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FcRn (FCGRT) in 10 µg of mouse liver tissue lysate.

Target description

~53 kDa observed; 40.09 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1