ABC148

Anti-TMEM132A Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Transmembrane protein 132A, HSPA5-binding protein 1, TMEM132A
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, rat, mouse
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• NCBI accession no.: NP_060340
• UniProt accession no.: Q24JP5
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TMEM132A(54972)

Description

General description

Transmembrane protein 132A (TMEM132A), or HSPA5-binding protein 1 (HSPA5BP1), is thought to be involved in embryonic and postnatal development of the brain, and the increased cell death resistance induced by serum starvation in cultured cells. TMEM132A is known to regulate cAMP-induced GFAP gene expression through phosphorylation of STAT3.

~140 kDa observed. The calculated molecular weight of this protein is 110kDa This protein has been observed at ~135 and ~170 kDa due to glycosylation, myristoylation, and phosphorylation (Kentaro, O. H., et al. (2003). J Biol Chem. 278(12): 10531-10537.). Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TMEM132A.

Application

Detect TMEM132A using this rabbit polyclonal antibody, Anti-TMEM132A Antibody validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Neuro2A in human cerebellum and thalamus tissues.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

This antibody recognizes the cytoplasmic domain of TMEM132A.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Neuro2A cell lysate

Evaluated by Western Bloting in Neuro2A cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected TMEM132A in 10 µg of Neuro2A cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable