ABC12

Anti-Bak (NT) Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, pro-apoptotic protein BAK
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IHC, IP, WB
• Citations: 3

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, rat, human
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_001179
• UniProt accession no.: Q16611
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BAK1(578)

Description

General description

Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.

~ 24 kDa

Immunogen

KLH-conjugated linear peptide corresponding to human BAK at and around the N-terminus.

Application

Detect Bak (N-terminus) using this Anti-Bak (N-terminus) Antibody validated for use in WB, IH, IC & IP.

Western Blot (SNAP ID) Analysis: 1 - 4 µg/ml from a previous lot detected Bak on 10 µg of Hek293 cell lysate.

Immunoprecipitation Analysis: For unpurified antibodies. 10 µg from a previous lot immunoprecipitated Bak from 500 µg of NIH3T3 lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Bak in kidney tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Bak in HeLa, NIH/3T3, and A431 cells.

Biochem/physiol Actions

This antibody recognizes BAK.

Analysis Note

Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Bak on 10 µg of Hek293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-433

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable