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650357

Sigma-Aldrich

Trypsin Inhibitor, Soybean, High Activity

Trypsin Inhibitor, Soybean, High Activity, CAS 9035-81-8 is of the several protease inhibitors in soybeans. Inhibits trypsin in 1:1 stoichiometry.

Synonym(s):

Trypsin Inhibitor, Soybean, High Activity

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About This Item

CAS Number:
9035-81-8
UNSPSC Code:
12352202
NACRES:
NA.28

Quality Level

300

Assay

≥90% (SDS-PAGE)

form

lyophilized solid

specific activity

≥7,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

color

white

solubility

67 mM potassium phosphate buffer, pH 7.6: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

Component VI of Rackis. Does not inhibit chymotrypsin activity.
Inactivates trypsin on an equal molar basis. To ensure complete inhibition of trypsin activity, a working concentration of soybean trypsin inhibitor should exceed that of trypsin by a factor of at least 2.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Trypsin
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of protein that will inhibit 1 unit of trypsin activity using BAEE as a substrate at 25°C, pH 7.6.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Uchino, T., et al. 1993. J. Biol. Chem.268, 527.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10-13 - German Storage Class 10 to 13

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ke Liu et al.
Cellular and molecular gastroenterology and hepatology, 13(2), 483-500 (2021-09-26)
Pancreatitis is characterized by acinar cell death and persistent inflammation. Ferroptosis is a type of lipid peroxidation-dependent necrosis, which is negatively regulated by glutathione peroxidase 4. We studied how trypsin, a serine protease secreted by pancreatic acinar cells, affects the

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