04-293

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8

clone 7F8, Upstate^®, from mouse

• Synonym(s): NEU, NGL, HER2, TKR1, HER-2, c-erb B2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 7F8, monoclonal
• Species reactivity: mouse, human, canine
• Application: ELISA, WB
• Citations: 5

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7F8, monoclonal
• species reactivity: mouse, human, canine
• manufacturer/tradename: Upstate^®
• technique(s): ELISA: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001005862.1, NM_004448.2
• UniProt accession no.: P04626
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr686)
• Gene Information: human ... ERBB2(2064) ; mouse ... Erbb2(13866)

Description

General description

180 kDa

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2

Application

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Kinases & Phosphatases

Biochem/physiol Actions

Recognizes phosphorylated erbB2 on Threonine 686

Physical form

Format: Purified

In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.

Subsequent thiophilic adsorption and size exclusion chromatography

Preparation Note

2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Routinely evaluated by immunoblot.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids