A new fluorometric assay for determining dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) was developed. The synthetic substrate glycyl-L-proline-4-methoxy-2-naphthylamide (20 mmol/L), Tris buffer (50 mmol/L, pH 8.3), and serum (20 microL) are mixed and incubated. The reaction is stopped with
Journal of pharmaceutical sciences, 86(3), 378-383 (1997-03-01)
Metabolism studies on organized HaCaT keratinocyte cell sheets are reported. Cells were grown on porous membranes to form organized cell sheets of several cell layers, which were considered as a model of viable epidermis. Metabolism was studied by reflection kinetics
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 33(7), 677-686 (1985-07-01)
Dipeptidyl peptidase II (DPP II) in normal rat lung was evaluated by the enzymes' ability to hydrolyze Lys-Ala or Lys-Pro derivatives of 4-methoxy-2-naphthylamine (MNA). For visualization of this activity, the liberated MNA was coupled with fast blue B for light
European journal of biochemistry, 235(3), 564-573 (1996-02-01)
Current methods to estimate changes in intralysosomal pH in hepatocytes do not discriminate between lysosomes and other intracellular acidic compartments. To obtain selective information on the change in lysosomal function in response to a change in lysosomal pH we have
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(3A Suppl), 464-480 (1981-03-01)
Aminopeptidase (AP) A, B, and M, gamma-glutamyltranspeptidase (GGT), endopeptidase I and II, membrane-associated endopeptidase I and II, dipeptidylaminopeptidase (DAP) I, II, and IV, trypsin and chymotrypsin were investigated with 4-methoxy-2-naphthylamine (MNA) substrates and ester proteinases with n-acetyl-L-methionine-1-naphthylester as substrate in
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