Classical Plant Media Formulations

Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today. Below, find formulation information for these commonly used media.

Component (mg/L)	C1416	D6162	G5768	G5893	H2395	M6774	M5524	Q3376	S6765	W0876
Aluminum chloride • 6H2O
Ammonium nitrate		1416.0				400.0	1650.0	400.0
Ammonium phosphate monobasic					115.03				300.0
Ammonium sulfate	463.0		134.0	134.0
Boric acid	1.6	4.8	3.0	3.0	2.86	6.2	6.2	6.2	5.0	1.5
Calcium chloride anhydrous	125.33	112.5	113.24	113.24		72.5	332.2		151.0
Calcium nitrate		1367.0			656.4	386.0		833.77		200.0
Calcium phosphate tribasic
Cobalt chloride • 6H2O			0.025	0.025			0.025	0.025	0.1
Cupric sulfate • 5H2O		0.25	0.025	0.025	0.08	0.25	0.025	0.025	0.2
Na2-EDTA	37.25	45.4	37.3	37.25		37.3	37.26	37.3	20.0
ZnNa2-EDTA
Ferric chloride • 6H2O
Ferric citrate
Ferric sulfate										2.5
Ferric tartrate • 2H2O					5.32
Ferrous sulfate • 7H2O	27.85	33.8	27.8	27.85		27.8	27.8	27.8	15.0
Magnesium sulfate	90.37	361.49	122.09	122.09	240.76	180.7	180.7	175.79	195.4	360.0
Manganese chloride • 4H2O					1.81
Manganese sulfate • H2O	3.33	33.5	10.0	10.0		22.3	16.9	0.76	10.0	5.04
Molybdenum trioxide					0.016
Molybdic acid (sodium salt) • 2H2O		0.39	0.25	0.25		0.25	0.25	0.25	0.1
Nickel chloride • 6H2O
Nickel sulfate • 6H2O		0.005
Potassium chloride										65.0
Potassium iodide	0.8		0.75	0.75			0.83	0.08	1.0	0.75
Potassium nitrate	2830.0		2500.0	2500.0	606.6		1900.0	1800.0	2500.0	80.0
Potassium phosphate monobasic	400.0	265.0				170.0	170.0	270.0
Potassium sulfate		1559.0				990.0
Sodium nitrate
Sodium phosphate monobasic			130.5	130.5						16.5
Sodium sulfate										200.0
Zinc nitrate • 6H2O		17.0
Zinc sulfate • 7H2O	1.5		2.0	2.0	0.22	8.6	8.6	8.6	1.0	2.67

Organics (mg/L)	C1416	D6162	G5768	G5893	H2395	M6774	M5524	Q3376	S6765	W0876
Activated charcoal
Adenine hemisulfate
Agar
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma- Dimethylallylaminopurine
Glycine (free base)
Indole-3-acetic acid
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol				100.0
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid)				1.0
Peptone
Pyridoxine • HCl				1.0
Sucrose
Thiamine • HCl				10.0

	C1416	D6162	G5768	G5893	H2395	M6774	M5524	Q3376	S6765	W0876
Grams of powder to prepare 1 L	4.0	5.2	3.1	3.2	1.6	2.3	4.3	3.6	3.2	0.934
pH ± 0.5 at room temperature	4.1	4.1	4.0	4.0	4.7	3.9	3.9	3.9	4.2	4.7