LiChrospher^® HPLC Columns

Section Overview:

• LiChrospher^® 100 RP-18 and RP-18 endcapped
• LiChrospher^® 100 RP-8 and RP-8 endcapped
• LiChrospher^® 60 RP-Select B
• LiChrospher^® 100 DIOL
• LiChrospher^® 100 NH2
• LiChrospher^® 100 CN
• LiChrospher^® Si 60 and Si 100
• LiChrospher^® PAH

LiChrospher^® HPLC columns are known for their reliable, fully porous spherical silica-based sorbent, which is recognized for its trusted performance in thousands of HPLC methods throughout various application fields. The sorbent is produced traditionally with various modifications, allowing it to adapt to different chromatographic needs.

The non-polar modified phases LiChrospher^® RP-8, RP-8 endcapped, RP-select B, RP-18, RP-18 endcapped are made for reversed-phase separations.

For the normal phase the polar modified phases, such as LiChrospher^® CN, LiChrospher^® NH2, and LiChrospher^® DIOL, as well as the unmodified LiChrospher^® SI, are well-suited.

Additionally, LiChrospher^® PAH is renowned for its high selectivity in separating polycyclic aromatic hydrocarbons (PAHs).

LiChrospher^® packing materials are available in Hibar^® RT ready-to-use columns and LiChroCART^® cartridges, offering a variety of lengths and internal diameters (10 mm, 4.6 mm, 4 mm, 3 mm, and 2 mm). The narrow-bore LiChroCART^® cartridges (3 mm I.D. and 2 mm I.D.) offer significant benefits for HPLC by minimizing solvent consumption, decreasing operational costs, and improving sensitivity and resolution. Additionally, LiChroCART^® cartridges with internal diameters of 4.6 mm, 4 mm, 3 mm, and 2 mm are compatible with the manu-CART^® NT system, enabling faster and more adaptable method adjustments for smaller bore columns. The LiChroCART^® cartridges, with a 10 mm internal diameter, are specifically designed for larger-scale applications and are compatible with the manu-CART^® “10”-II system.

Table 1. Specifications of LiChrospher^® packing materials

Packing material	USP Designation	Characteristics/Phase	Pore size (Å)	Particle size (µm)	% C	Endcapped
LiChrospher® 100 RP-18	L1	Spherical particles of silica with octadecyl derivative	100	5, 10	21.0	no
LiChrospher®RP-18 endcapped	L1	Spherical particles of silica with octadecyl derivative endcapped	100	5, 10	21.6	yes
LiChrospher® PAH	L1	Spherical particles of silica with octadecyl derivative	150	5	proprietary	no
LiChrospher® 100 RP-8	L7	Spherical particles of silica with octyl derivative	100	5, 10	12.5	no
LiChrospher® 100 RP-8 endcapped	L7	Spherical particles of silica with octyl derivative endcapped	100	5, 10	13.0	yes
LiChrospher® RP-select B	L7	Octylsilane deactivated for the separation of basic compounds	60	5, 10	11.5	no
LiChrospher® 100 DIOL	L20	Spherical particles of silica with vicinal hydroxyl function on C-chains	100	5	8.0	no
LiChrospher® 100 NH2	L8	Spherical particles of silica with aminopropyl function	100	5	4.6	no
LiChrospher® 100 CN	L10	Spherical particles of silica with cyanopropyl function	100	5, 10	6.6	no
LiChrospher® Si 60	L3	Spherical particles of silica	60	5, 10	n.a.	n.a.
LiChrospher® Si 100		Spherical particles of silica	100	5	n.a.	n.a.
LiChrospher® 60 RP-select B		Spherical particles of silica with octyl derivative, especially suitable for the RP separation of basic compounds	60	5,10	11.5	no

LiChrospher^® 100 RP-18 and RP-18 endcapped HPLC Columns

LiChrospher^® 100 RP-18 and RP-18 endcapped HPLC columns are very reliable and highly versatile C18 phases. These traditionally manufactured spherical silica-based supports are intended for reversed-phase chromatography of acidic, neutral, and weakly basic compounds—substances commonly encountered across various analytical fields. Several individual batches are utilized to create a large master batch of the LiChrospher^® RP-18 sorbent. This removes discrepancies among various small batches and guarantees the sorbent's outstanding consistency from one batch to another. As a result, LiChrospher^® RP-18 provides consistently reproducible outcomes, ensuring the reliability of your HPLC method.

LiChrospher^® RP-18 HPLC Columns are classified as USP L1 columns, meeting the stringent requirements for reversed-phase chromatography and ensuring consistent, high-quality separations in pharmaceutical and analytical applications.

Specifications of LiChrospher^® 100 RP-18 and RP-18 endcapped HPLC Columns

	LiChrospher® 100 RP-18	LiChrospher® 100 RP-18 endcapped
Sorbent characteristics	Particles of silica with octadecyl derivative	Particles of silica with octadecyl derivative endcapped
Particle shape	Fully porous spherical silica particles (Type A)	Fully porous spherical silica particles (Type A)
Particle size	5, 10 μm	5, 10 μm
Pore size	100 Å (10 nm)	100 Å (10 nm)
Pore volume	1.25 mL/g	1.25 mL/g
Spec. surface area	350 m2/g	350 m2/g
Carbon load	21.0 % C	21.6 % C
Coverage of the surface	4,09 μmol/m2	4.09 μmol/m2
Minimum Efficiency	50,000 N/m (5 µm); 25,000 N/m (10 µm)	55,000 N/m (5 µm); 25,000 N/m (10 µm)
pH range	2-7.5	2-7.5
Shipping eluent	Acetonitrile/water	Acetonitrile/water

LiChrospher^® 100 RP-8 and RP-8 endcapped HPLC Columns

LiChrospher^® 100 RP-8 and RP-8 endcapped HPLC columns are made from spherical silica gel carriers that exhibit C8 or C8 endcapped reversed-phase characteristics. The columns demonstrate exceptional versatility, making them appropriate for acidic, neutral, and weakly basic compounds. The columns provide excellent selectivity and performance, guaranteeing consistent separations across different batches and over the years.

Specifications of LiChrospher^® 100 RP-8 and RP-8 endcapped

	LiChrospher® 100 RP-8	LiChrospher® 100 RP-8 endcapped
Sorbent characteristics	Particles of silica with octyl derivative	Particles of silica with octyl derivative endcapped
Particle shape	Fully porous spherical silica particles (Type A)	Fully porous spherical silica particles (Type A)
Particle size	5, 10 μm	5, 10 μm
Pore size	100 Å (10 nm)	100 Å (10 nm)
Pore volume	1.25 mL/g	1.25 mL/g
Spec. surface area	350 m2/g	350 m2/g
Carbon load	12.5 % C	13 % C
Coverage of the surface	4.04 μmol/m2	4.44 μmol/m2
Minimum Efficiency	55,000 N/m (5 µm); 25,000 N/m (10 µm)	55,000 N/m (5 µm); 25,000 N/m (10 µm)
pH range	2-7.5	2-7.5
Shipping eluent	Acetonitrile/water	Acetonitrile/water

LiChrospher^® 60 RP-Select B HPLC Columns

LiChrospher^® 60 RP-Select B is designed specifically for HPLC separations of basic compounds. The sorbent, derived from spherical silica particles, effectively prevents secondary interactions with basic substances, ensuring their elution as highly symmetrical peaks. In addition to providing outstanding separation properties for basic compounds, LiChrospher^® 60 RP-Select B is also appropriate for the analysis of neutral and acidic substances. For LiChrospher^® RP-select B, multiple individual batches are utilized to create a large master batch of the sorbent. This removes discrepancies among various small batches and guarantees the sorbent's outstanding consistency from one batch to another. As a result, LiChrospher^® RP-select B column provides exceptionally consistent outcomes, ensuring the dependability of your HPLC method.

The exceptional reproducibility of the “master batch LiChrospher^® RP-select B" in comparison to smaller individual batches of the sorbent is clearly evidenced by the Tanaka test, a rigorous HPLC column testing procedure.

Specifications of LiChrospher^® 60 RP-Select B

Sorbent characteristics	Particles of silica with octyl derivative
Particle shape	Fully porous spherical silica particles (Type A)
Particle size	5 μm
Pore size	60 Å (6 nm)
Pore volume	0.9 mL/g
Spec. surface area	360 m2/g
Carbon load	11.5 % C
Coverage of the surface	3.55 μmol/m2
Minimum Efficiency	55,000 N/m;
pH range	2-7.5
Shipping eluent	Acetonitrile/water

LiChrospher^® 100 DIOL HPLC Columns

LiChrospher^® 100 DIOL has both polar and hydrophobic characteristics, combining weak hydrophobic interactions with polar interactions. This allows for successful HPLC separation of complex samples. The sorbent can be employed as a less polar alternative to LiChrospher^® Si 60 in normal-phase applications, as well as a less hydrophobic alternative (with some limits) to LiChrospher^® RP-8 in reversed-phase applications. Furthermore, LiChrospher^® DIOL is suitable for HILIC chromatography.

Specifications of LiChrospher^® 100 DIOL

Sorbent characteristics	Particles of silica with diol function on C-chains
Particle shape	Fully porous spherical silica particles (Type A)
Particle size	5 μm
Pore size	100 Å (10 nm)
Pore volume	1.25 mL/g
Spec. surface area	350 m2/g
Carbon load	8.0 % C
Coverage of the surface	3.87 μmol/m2
Minimum Efficiency	40,000 N/m
pH range	2-7.5
Shipping eluent	n-Heptane

LiChrospher^® 100 NH2 HPLC Columns

LiChrospher^® NH2 is a versatile sorbent that features both polar and hydrophobic characteristics. This makes it appropriate for normal-phase, reversed-phase, HILIC, and ion exchange chromatography. Common applications involve the separation of carbohydrates (mono-, di-, and oligosaccharides) using reversed-phase chromatography, as well as the separation of nucleotides through ion exchange chromatography. 

Specifications of LiChrospher^® 100 NH2

Sorbent characteristics	Particles of silica with gamma-aminopropyl function
Particle shape	Fully porous spherical silica particles (Type A)
Particle size	5 μm
Pore size	100 Å (10 nm)
Pore volume	1.25 mL/g
Spec. surface area	350 m2/g
Carbon load	4.6 % C
Coverage of the surface	4.1 μmol/m2
Minimum Efficiency	25000 N/m
pH range	2-7.5
Shipping eluent	n-Heptane

LiChrospher^® 100 CN HPLC Columns

LiChrospher^® 100 CN exhibits a unique combination of polar and hydrophobic properties, integrating weak hydrophobic interactions alongside polar interactions. This facilitates effective HPLC separations of intricate samples. The sorbent is a less polar option than LiChrospher^® Si 60 for normal-phase applications or a less hydrophobic choice relative to LiChrospher^® RP-8 for reversed-phase applications. This adaptable sorbent is also appropriate for selective charged interactions.

Specifications of LiChrospher^® 100 CN

Sorbent characteristics	Particles of silica with gamma-cyanopropyl function
Particle shape	Fully porous spherical silica particles (Type A)
Particle size	5, 10 μm
Pore size	100 Å (10 nm)
Pore volume	1.25 mL/g
Spec. surface area	350 m2/g
Carbon load	6.6 % C
Coverage of the surface	3.52 μmol/m2
Minimum Efficiency	40,000 N/m (5 µm); 15,000 N/m (10 µm)
pH range	2-7.5
Shipping eluent	n-Heptane

LiChrospher^® Si 60 and Si 100 HPLC Columns

LiChrospher^® Si 60 columns and Si 100 are versatile HPLC columns with superior polar characteristics for normal-phase separations. They use spherical silica particles with pore diameters of 60 or 100 Å and particle sizes of 5 or 10 µm.

Specifications of LiChrospher^® Si 60 and Si 100

	LiChrospher® Si 60	LiChrospher® Si 100
Sorbent characteristics	Particles of silica	Particles of silica
Particle shape	Fully porous spherical silica particles (Type A)	Fully porous spherical silica particles (Type A)
Particle size	5, 10 μm	5 μm
Pore size	60 Å (10 nm)	100 Å (10 nm)
Pore volume	0.85 mL/g	1.25 mL/g
Spec. surface area	700 m2/g	400 m2/g
Minimum Efficiency	55,000 N/m (5 µm); 20,000 N/m (10 µm)	55,000 N/m
pH range	2-7.5	2-7.5
Shipping eluent	n-Heptane	n-Heptane

LiChrospher^® PAH HPLC Columns

LiChrospher^® PAH is a highly selective HPLC column, built specifically for the trace analysis of PAH. The column delivers outstanding, high-resolution gradient separations of 16 PAH in accordance with EPA 610 and EPA 550, in addition to benzo[e]pyrene and perylene. Further, the LiChrospher^® PAH columns are well-suited for the isocratic separation of 6 PAHs in accordance with the German DIN method. They incorporate programmed fluorescence detection, enabling precise and sensitive analysis. During typical runs, the first eluting polycyclic aromatic hydrocarbon (PAH), naphthalene, appears at approximately 10 minutes, with full separation completed within 30 minutes.

Specifications of LiChrospher^® PAH HPLC Columns

Sorbent characteristics	Particles of silica with octadecyl derivative
Particle shape	Fully porous spherical silica particles (Type A)
Particle size	5 μm
Pore size	150 Å (15 nm)
Spec. surface area	200 m2/g
Carbon load	20 %
pH range	2-7.5
Shipping eluent	Acetonitrile/water

Ordering Information

LiChrospher^® HPLC Columns with 5 µm Particle Size in column hardware LiChroCART^® Cartridge (manu-CART^® required)

Column dimension
Length (mm)		ID (mm)	RP-18 (5 µm)	RP-18 endcapped (5 µm)	PAH (5 µm)	RP-8 (5 µm)	RP-8 endcapped (5 µm)	RP-select B (5 µm)	Diol (5 µm)	CN (5 µm)	NH2 (5 µm)	Si 60 (5 µm)	Si 100 (5 µm)
25	x	4	1.50931.0001*	1.50936.0001*		1.50930.0001*	on request	1.50937.0001*
55	x	2
75	x	4	1.50987.0001*	on request			on request	1.50993.0001*
100	x	4.6	1.50600.0001	1.50603.0001		1.50634.0001	1.50637.0001	1.50640.0001
125	x	3	1.50159.0001			on request	on request
125	x	4	1.50823.0001	1.50828.0001		1.50822.0001	1.50827.0001	1.50829.0001	1.50826.0001	1.50825.0001	1.50824.0001	1.50820.0001
125	x	4	1.50943.0001*	1.50734.0001*		1.50942.0001*	on request	1.50981.0001*
125	x	4.6	on request	1.51908.0001		on request	on request	on request
150	x	4.6	1.50601.0001	1.50604.0001		1.50635.0001	1.50638.0001	1.50641.0001
250	x	3	1.50154.0001	on request	1.50156.0001	on request	on request	1.50155.0001
250	x	4	1.50833.0001	1.50838.0001	1.50149.0001		1.50837.0001	1.50839.0001	1.50836.0001	1.50892.0001	1.50834.0001	1.50830.0001
250	x	4	1.50983.0001*	1.50995.0001*		1.50982.0001*	on request	1.50984.0001*
250	x	4.6	1.50602.0001			1.50636.0001	1.50639.0001
100	x	4.6	1.50600.1003			1.50634.1003		1.50640.1003
125	x	3	1.50159.1003
125	x	4	1.50823.1003			1.50822.1003		1.50981.1003
125	x	4.6
150	x	4.6						1.50641.1003
250	x	3	1.50154.1003					1.50155.1003
250	x	4	1.50833.1003					1.50839.1003
250	x	4.6	1.50602.1003			1.50636.1003
4	x	4	1.50957.0001	1.50962.0001	1.50148.0001	1.50956.0001	1.50961.0001	1.50963.0001	1.50960.0001	1.50959.0001	1.50958.0001	1.50955.0001
* 3 units The LiChroCART® columns (75, 100, 125, 150, and 250 mm length) in the list above (2, 3, 4, and 4.6 mm I.D.) require part number 1.51486.0001 manu-CART® cartridge column holder, which can be used to hold one cartridge column with or without a 4-4 mm guard column. LiChroCART® columns 250-10 mm require part number 1.51419.0001 manu-CART® 10. Additional dimensions and validation kits are available as customized packings on request.

LiChrospher^® HPLC Columns with 5 µm Particle Size in Hibar^® RT column hardware

Column dimension
Length (mm)		ID (mm)	RP-18 (5 µm)	RP-18 endcapped (5 µm)	PAH (5 µm)	RP-8 (5 µm)	RP-8 endcapped (5 µm)	RP-select B (5 µm)	Diol (5 µm)	CN (5 µm)	NH2 (5 µm)	Si 60 (5 µm)	Si 100 (5 µm)
Hibar® RT HPLC Column [1 unit]
50	x	4
100	x	4.6	1.50545.0001			1.50578.0001	1.50581.0001	1.50573.0001
125	x	2	on request	1.51907.0001		on request	on request	on request
125	x	4	1.50477.0001	on request		on request	on request	on request
125	x	4.6	on request	1.51906.0001		on request	on request	on request
150	x	3
150	x	4.6	1.50546.0001	1.50549.0001		1.50579.0001	1.50582.0001	1.50574.0001			1.51905.0001
250	x	3
250	x	4	1.50377.0001	on request	on request	1.50329.0001	on request	on request					1.50316.0001
250	x	4.6	1.50547.0001	1.50550.0001		1.50580.0001	1.50583.0001	1.50575.0001			1.51904.0001
Validation Kits [3 Hibar® RT HPLC Columns from 3 different sorbent batches]
100	x	4.6	1.50545.1003			1.50578.1003		1.50573.1003
125	x	4	1.50477.1003
150	x	4.6		1.50549.1003				1.50574.1003
250	x	4	1.50377.1003			1.50329.1003
240	x	4.6		1.50550.1003
The Hibar® columns are complete with endfittings. When using a guard column with a Hibar® column, we recommend part number 1.51487.0001 guard column cartridge holder for 4-4 mm guard column LiChroCART® cartridges. Additional dimensions are available as customized packings on request.

LiChrospher^® HPLC Columns with 10 µm Particle Size in column hardware LiChroCART^® Cartridge (manu-CART^® required)

Column dimension
Length (mm)		ID (mm)	RP-18 (10 µm)	RP-18 endcapped (10 µm)	RP-8 (10 µm)	RP-8 endcapped (10 µm)	RP-select B (10 µm)	Diol (10 µm)	CN (10 µm)	NH2 (10 µm)	Si 60 (10 µm)
LiChroCART® HPLC Cartridge [1 unit; * 3 units]
10	x	10
250	x	4	1.50843.0001	1.50848.0001	1.50842.0001	1.50847.0001	1.50742.0001*	on request	1.50845.0001		1.50840.0001
250	x	4.6
250		10	1.50853.0001	1.50858.0001	on request		on request	on request	on request	on request	1.50850.0001
The LiChroCART® columns (75, 100, 125, 150, and 250 mm length) in the list above (2, 3, 4, and 4.6 mm I.D.) require part number 1.51486.0001 manu-CART® cartridge column holder, which can be used to hold one cartridge column with or without a 4-4 mm guard column. LiChroCART® columns 250-10 mm require part number 1.51419.0001 manu-CART® 10. Additional dimensions and validation kits are available as customized packings on request.

LiChrospher^® HPLC Columns with 10 µm Particle Size in Hibar^® RT Column Hardware are available on request.