In vitro evaluation of osteoconductivity and cellular response of zirconia and alumina based ceramics.

In vitro evaluation of osteoconductivity and cellular response of zirconia and alumina based ceramics.

Materials science & engineering. C, Materials for biological applications (2013-08-06)

Ajoy Kumar Pandey, Falguni Pati, Debika Mandal, Santanu Dhara, Koushik Biswas

ZUSAMMENFASSUNG

Developed ceria/yttria stabilized zirconia and ceria/yttria stabilized zirconia toughened alumina supported formation of apatite layer when immersed in simulated body fluid without any prior surface treatment. The formed mineral layer was confirmed as hydroxyapatite through X-ray diffraction patterns. The calcium/phosphate atomic ratio obtained from energy dispersive X-ray spectroscopy was found to be little less (Ca/P=1.5) than that of pure hydroxyapatite (Ca/P=1.7) which indicates the probability of mixed type calcium-phosphate compound formation. The achieved thickness of apatite layer was estimated through a surface profilometer and as high as ~17 μm thickness was found after 28 days of soaking. The biocompatibility of the developed materials was ensured through in vitro human osteoblast like cell (MG63) culture on ceramic discs. The morphology of attached cells was characterized through scanning electron microscopy and fluorescent microscopy which show multilayered interconnected cell growth within 8 days of culture period. Moreover, differentiation of MG63 cells was evaluated through MTT assay, total protein content and alkaline phosphatase activity.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

199443

Sigma-Aldrich

Aluminiumoxid, Brockmann I, standard grade, activated, basic

199974

Sigma-Aldrich

Aluminiumoxid, activated, Brockmann I, standard grade, neutral

230693

Sigma-Aldrich

Zirkonium(IV)-oxid, powder, 5 μm, 99% trace metals basis

229423

Sigma-Aldrich

Aluminiumoxid, powder, 99.99% trace metals basis

199966

Sigma-Aldrich

Aluminiumoxid, Brockmann I, standard grade, activated, acidic

544833

Sigma-Aldrich

Aluminiumoxid, nanopowder, <50 nm particle size (TEM)

544841

Sigma-Aldrich

Cer(IV)-oxid, nanopowder, <25 nm particle size (BET)

544760

Sigma-Aldrich

Zirkonium(IV)-oxid, nanopowder, <100 nm particle size (TEM)

702129

Sigma-Aldrich

Aluminiumoxid, nanoparticles, <50 nm particle size (DLS), 20 wt. % in isopropanol

211575

Sigma-Aldrich

Cer(IV)-oxid, powder, <5 μm, 99.9% trace metals basis

234745

Sigma-Aldrich

Aluminiumoxid, Corundum, α-phase, -100 mesh

22390

Sigma-Aldrich

Cer(IV)-oxid, ≥99.0%

700290

Sigma-Aldrich

Cer(IV)-oxid, nanopowder, <50 nm particle size (BET), 99.95% trace rare earth metals basis

342688

Sigma-Aldrich

Aluminiumoxid, fused, powder, primarily α-phase, -325 mesh

718475

Sigma-Aldrich

Aluminiumoxid, nanopowder, 13 nm primary particle size (TEM), 99.8% trace metals basis

403296

Sigma-Aldrich

Zirconium, powder, −100 mesh

202606

Sigma-Aldrich

Aluminiumoxid, 99.997% trace metals basis

A1522

Sigma-Aldrich

Aluminiumoxid, Type WN-6, Neutral, Activity Grade Super I

267740

Sigma-Aldrich

Aluminiumoxid, pore size 58 Å, ~150 mesh

642991

Sigma-Aldrich

Aluminiumoxid, nanoparticles, 30-60 nm particle size (TEM), 20 wt. % in H₂O

643009

Sigma-Aldrich

Cerium(IV)-oxid, Dispersion -Lösung, nanoparticles, <25 nm particle size, 10 wt. % in H₂O

202975

Sigma-Aldrich

Cer(IV)-oxid, powder, 99.995% trace metals basis

342653

Sigma-Aldrich

Aluminiumoxid, fused, powder, primarily α-phase, 100-200 mesh

204994

Sigma-Aldrich

Zirkonium(IV)-oxid, 99.99% trace metals basis (purity excludes ~2% HfO₂)

419141

Sigma-Aldrich

Zirconium, foil, thickness 0.1 mm, 99.98% trace metals basis

634875

Sigma-Aldrich

Aluminiumoxid, single crystal substrate, <0001>

551643

Sigma-Aldrich

Aluminiumoxid, nanowires, diam. × L 2-6 nm × 200-400 nm

517747

Sigma-Aldrich

Aluminumoxid, mesoporös, MSU-X (wormhole), average pore size 3.8 nm

262994

Sigma-Aldrich

Yttrium, chips, 99.9% trace rare earth metals basis

267724

Sigma-Aldrich

Zirconium, rod, diam. 6.35 mm, ≥99% trace metals basis