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  • Identification of selenium species in selenium-enriched Lens esculenta plants by using two-dimensional liquid chromatography-inductively coupled plasma mass spectrometry and [77Se]selenomethionine selenium oxide spikes.

Identification of selenium species in selenium-enriched Lens esculenta plants by using two-dimensional liquid chromatography-inductively coupled plasma mass spectrometry and [77Se]selenomethionine selenium oxide spikes.

Journal of chromatography. A (2006-12-05)
Zoyne Pedrero, Jorge Ruiz Encinar, Yolanda Madrid, Carmen Cámara
ZUSAMMENFASSUNG

Selenium speciation in Se-enriched Lens esculenta grown in hydroponic culture containing inorganic selenium as Na(2)SeO(3) and Na(2)SeO(4) was performed. After 16 days of growth, the plants were collected and divided in two parts, roots and stems and then analysed to identify and quantify selenium species. Speciation studies of the enzymatic extracts were carried out by using anion-exchange (PRP-X100) and size-exclusion/ion-exchange (Shodex Asahipak) columns coupled to inductively coupled plasma mass spectrometry (ICP-MS). The need of using two independent chromatographic mechanisms for unambiguous species identification is demonstrated. Moreover, the use of a [(77)Se]selenomethionine selenium oxide spike turned out to be critical to discriminate between selenium selenomethioine selenium oxide and selenocysteine.

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Produktbeschreibung

Sigma-Aldrich
Selendioxid, reagent grade, powder, 98%
Sigma-Aldrich
Selendioxid, ≥99.9% trace metals basis
Sigma-Aldrich
Selendioxid, 99.999% trace metals basis
Sigma-Aldrich
Selendioxid, ReagentPlus®, powder, 99.8% trace metals basis