Direkt zum Inhalt
Merck

Investigation of biomarker in laryngeal carcinomas.

Journal of clinical laboratory analysis (2014-01-08)
Deniz Kanliada, Ender Coskunpinar, Kadir Serkan Orhan, Yasemin Musteri Oltulu, Mehmet Celik, Ayse Eren, Ilhan Yaylim, Kemal Deger
ZUSAMMENFASSUNG

The aim of the study is to determine whether there is a role of podoplanin and glutathione S-transferases T1 (GST-T1) expression in laryngeal squamous cell carcinoma. In this study, 33 patients were enrolled and gene expression analysis was performed by qRT-PCR. The podoplanin and GST-T1 expression patterns were analyzed to determine their correlation with clinicopathologic parameters of laryngeal cancer. Of all included patients, 20 had supraglottic, and 13 had glottic laryngeal cancer. Increased expression of podoplanin was found in seven (35%) supraglottic tumor tissues and seven (53.8%) glottic tumor tissues, but GST-T1 expression was not detected. Podoplanin expression did not show any prediction for tumor differentiation, regional metastasis, thyroid cartilage invasion, lymphatic vessel invasion, or tumor differentiation for laryngeal cancer, and also there were no significant differences in podoplanin expression between glottic and supraglottic regions, but extracapsullar extension is almost statistically significance (P = 0.05).

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Roche
High Pure RNA-Gewebekit, sufficient for 50 isolation(s), suitable for RT-PCR, suitable for Northern blotting
Roche
First Strand cDNA-Synthesekit für die RT-PCR (AMV), sufficient for 30 reactions (including 5 control reactions), kit of 1 (10 components), suitable for RT-PCR, hotstart: no, dNTPs included